Abstract
Ovarian cancer (OVCA) is frequently detected at late stages of disease, often with dissemination throughout the peritoneal cavity surface, abdomen, and ascites fluid. Tumor signaling via mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K) pathways can promote OVCA progression and depend on local microenvironmental cues. To better study OVCA in situ within native tissue contexts, here we describe confocal microscopy techniques to image mouse models of intraperitoneal disease at a single-cell resolution. As a proof of principle demonstration, examples are highlighted for simultaneously imaging tumor vascularization, infiltrating and often immunosuppressive immune cells (tumor-associated macrophages), and OVCA kinase activity.
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Acknowledgments
This work was supported in part by NIH grants DP2CA259675 and R00CA207744. The authors thank the MGH Center for Systems Biology Mouse Imaging Program for experimental assistance.
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Matvey, D.O., Ng, T.S.C., Miller, M.A. (2022). Confocal Imaging of Single-Cell Signaling in Orthotopic Models of Ovarian Cancer. In: Kreeger, P.K. (eds) Ovarian Cancer. Methods in Molecular Biology, vol 2424. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1956-8_19
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DOI: https://doi.org/10.1007/978-1-0716-1956-8_19
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