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Ovarian Cancer

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Pamela K. Kreeger

    1. Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, USA

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2424)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Clinical Perspectives

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Clinical Staging of Ovarian Cancer

      • Andrea S. O’Shea
      Pages 3-10

    3. Pathologic Classification of Ovarian Cancer

      • Stephanie M. McGregor
      Pages 11-40

  3. Tumor Microenvironment

    1. Front Matter

      Pages 41-41
      Download chapter PDF

    2. Multiparameter Flow Cytometry for Detailed Characterization of Peritoneal Immune Cells from Patients with Ovarian Cancer

      • Jessica Vazquez, Dagna Sheerar, Aleksandar K. Stanic, Manish S. Patankar
      Pages 43-58

    3. Mass Cytometry for the Characterization of Individual Cell Types in Ovarian Solid Tumors

      • Veronica D. Gonzalez, Ying-Wen Huang, Wendy J. Fantl
      Pages 59-94

    4. Processing and Analysis of Ascites

      • Hannah M. Micek, Molly J. Carroll, Lisa Barroilhet, Pamela K. Kreeger
      Pages 95-104

    5. Multispectral Staining and Analysis of Extracellular Matrix

      • Carine M. Renner, Mike R. Visetsouk, Pamela K. Kreeger, Kristyn S. Masters
      Pages 105-119

    6. Quantitative Analysis of the Extracellular Matrix by Immunoblot

      • Kaitlin C. Fogg
      Pages 121-132

  4. Cell Isolation

    1. Front Matter

      Pages 133-133
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    2. Multiparameter Single-Cell Characterization of Ovarian Intratumor Heterogeneity

      • Kristin G. Beaumont, Christina Andreou, Ethan Ellis, Robert Sebra
      Pages 135-146

    3. Culturing Primary Human Mesothelial Cells

      • Mary Mullen, Hollie Noia, Katherine Fuh
      Pages 147-154

    4. Isolation of Normal and Cancer-Associated Fibroblasts

      • Katarzyna Zawieracz, Mark A. Eckert
      Pages 155-165

    5. Isolation of Primary Normal and Cancer-Associated Adipocytes from the Omentum

      • Abir Mukherjee
      Pages 167-175

  5. Model Systems

    1. Front Matter

      Pages 177-177
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    2. Isolation of Fallopian Tube Epithelium for Assessment of Cilia Beating Frequency (CBF)

      • Angela Russo, Joanna E. Burdette
      Pages 179-187

    3. Ex Vivo Ovarian Culture to Model the Initial Metastasis in Ovarian Cancer

      • Matthew Dean
      Pages 189-198

    4. In Vivo and Ex Vivo Analysis of Omental Adhesion in Ovarian Cancer

      • Elizabeth I. Harper, Tyvette S. Hilliard
      Pages 199-216

    5. The Role of the Tumor Microenvironment in CSC Enrichment and Chemoresistance : 3D Co-culture Methods

      • Michael Bregenzer, Eric Horst, Pooja Mehta, Catherine Snyder, Taylor Repetto, Geeta Mehta
      Pages 217-245
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Keywords

About this book

This detailed volume provides a robust set of methods to understand variation between patients with ovarian cancers, in vitro models to better study different stages of the disease, and in vivo models to test therapies. Beginning with clinical perspectives to orient basic scientists, the book continues with sections exploring methods to characterize features of the tumor microenvironment (TME), techniques to isolate cells for in vitro study, and in vitro and in vivo models to study the disease. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Ovarian Cancer: Methods and Protocols serves as an ideal guide for researchers seeking to improve their study of ovarian cancer and find newtherapeutic approaches.

Editors and Affiliations

  • Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, USA

    Pamela K. Kreeger

Bibliographic Information

  • Book Title: Ovarian Cancer

  • Book Subtitle: Methods and Protocols

  • Editors: Pamela K. Kreeger

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1956-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-1955-1Published: 17 December 2021

  • Softcover ISBN: 978-1-0716-1958-2Published: 18 December 2022

  • eBook ISBN: 978-1-0716-1956-8Published: 16 December 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 318

  • Number of Illustrations: 93 illustrations in colour

  • Topics: Cancer Research

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