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Establishment of In Vivo Ovarian Cancer Mouse Models Using Intraperitoneal Tumor Cell Injection

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Ovarian Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2424))

Abstract

Mouse models—xenograft models, syngeneic models (directly implanted or chemically or virally induced), and genetically engineered mice (including transgenic and knockout methods) are invaluable for preclinical studies of ovarian cancer as they recapitulate the structures and microenvironments of tumors, which in vitro studies are unable to accomplish.

This chapter describes the methodology and approaches for generating various murine models currently employed in ovarian cancer research. It covers the implantation of cells from ovarian cancer cell lines into mice by intraperitoneal injection.

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Author information

Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Medical College of Wisconsin, Milwaukee, WI, USA

    Sonam Mittal, Prachi Gupta, Pradeep Chaluvally-Raghavan & Sunila Pradeep

  2. Department of Obstetrics and Gynecology, Department of Physiology, Cancer Center, Medical College of Wisconsin, Milwaukee, WI, USA

    Pradeep Chaluvally-Raghavan & Sunila Pradeep

Authors
  1. Sonam Mittal
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  2. Prachi Gupta
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  3. Pradeep Chaluvally-Raghavan
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  4. Sunila Pradeep
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Corresponding author

Correspondence to Sunila Pradeep .

Editor information

Editors and Affiliations

  1. Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA

    Pamela K. Kreeger

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Mittal, S., Gupta, P., Chaluvally-Raghavan, P., Pradeep, S. (2022). Establishment of In Vivo Ovarian Cancer Mouse Models Using Intraperitoneal Tumor Cell Injection. In: Kreeger, P.K. (eds) Ovarian Cancer. Methods in Molecular Biology, vol 2424. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1956-8_16

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  • DOI: https://doi.org/10.1007/978-1-0716-1956-8_16

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1955-1

  • Online ISBN: 978-1-0716-1956-8

  • eBook Packages: Springer Protocols

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