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Applications of Impedance Flow Cytometry in Doubled Haploid Technology

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Doubled Haploid Technology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2289))

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Abstract

Efficient doubled haploid (DH) plant production is of great interest in the plant breeding industry and research because homozygous lines are obtained within a single generation shortening the breeding cycle substantially. DH protocol development can be a time- and resource-consuming process due to numerous factors affecting its success and efficiency. Here we present concepts and examples about how critical success factors can be identified throughout a DH protocol and an early microspore response monitored by simple impedance flow cytometry (IFC) measurements, which will help to optimize each step of an androgenesis-based DH protocol.

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Authors and Affiliations

  1. Acepo, Enkhuizen, The Netherlands

    Iris Heidmann

  2. Amphasys AG, Technopark Lucerne, Root, Switzerland

    Marco Di Berardino

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  1. Iris Heidmann
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  2. Marco Di Berardino
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Correspondence to Iris Heidmann .

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  1. Cell Biology Group — COMAV Institute, Universitat Politècnica de València, Valencia, Spain

    Jose M. Segui-Simarro

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Heidmann, I., Di Berardino, M. (2021). Applications of Impedance Flow Cytometry in Doubled Haploid Technology. In: Segui-Simarro, J.M. (eds) Doubled Haploid Technology. Methods in Molecular Biology, vol 2289. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1331-3_3

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  • DOI: https://doi.org/10.1007/978-1-0716-1331-3_3

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