Skip to main content

Advances in Microspore Culture Technology: A Biotechnological Tool for the Improvement of Medicinal Plants

  • Chapter
  • First Online:
Biotechnology for Medicinal Plants

Abstract

Doubled haploidy techniques are well established for some species and are routinely used for practical application and basic research. Haploid/doubled haploid plants can be produced via a number of ways although the in vitro methods of androgenesis (culture of the male gametophyte) or gynogenesis (culture of the female gametophyte) are the most common. For medicinal plants, very little genetic improvement has been undertaken when compared to major crop species. However, callus, embryos, and doubled haploid plants have been produced in several medicinal species using a range of media formulations and culture conditions. This chapter focuses on doubled haploid production from the male gametophyte and the factors influencing this response.Doubled haploidy methodology would be beneficial for medicinal species as uniform, homozygous, true breeding lines would be available for growers. Uniformity in plants and in the active ingredient is very important for regulatory standards and for those conducting clinical trials.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

  • Augustine AC, Nivas S, D’Souza L (2008) Induction of embryos and plantlets from anthers of Curculigo orchioides Gaertn. An endangered medicinal herb. Indian J Biotechnol 7:536–540

    CAS  Google Scholar 

  • Badea E, Iordan M, Mihalea A (1985) Induction of androgenesis in anther culture of Digitalis lanata. Revue Roumaine de biologie. Ser Biol Veg 30:63–71

    Google Scholar 

  • Bapat VA, Wenzel G (1982) In vitro haploid plantlet induction in Physalis ixocarpa Brot. through microspore embryogenesis. Plant Cell Rep 1:154–156

    Article  Google Scholar 

  • Barinova J, Clement C, Marting L, Baillieul F, Soukupova H, Heberle-Bors E, Touraev A (2004) Regulation of developmental pathways in cultured microspores of tobacco and snapdragon by medium pH. Planta 219:141–146

    Article  PubMed  CAS  Google Scholar 

  • Bastar MT, Bohanec B (2006) Screening for gynogenic ability in selected medicinal plant species: progress report. In: Touraev A, Forster B (eds) Haploids in higher plants III, Vienna, Feb 12–15 2006, p 50

    Google Scholar 

  • Bicknell RA, Borst NK (1996) Isolation of reduced genotypes of Hieracium pilosella using anther culture. Plant Cell, Tissue Organ Cult 45:37–41

    Article  Google Scholar 

  • Blakeslee AF, Belling J, Farnham ME, Bergner AD (1922) A haploid mutant in the Jimson weed, Datura stramonium. Science 55:646–647

    Article  PubMed  CAS  Google Scholar 

  • Bohanec B (1997) Haploid induction in buckwheat (Fagopyrum esculentum Moench). In: Jain SM, Sopory SK, Veilleux RE (eds) In Vitro Haploid Production in Higher Plants. Kluwer Academic Publishers, Dordrecht, pp 163–170

    Google Scholar 

  • Bohanec B (2006) Mechanisms of gynogenesis. In: Touraev A, Forster B (eds) Haploids in higher plants III, Vienna, Feb 12–15 2006, p 18

    Google Scholar 

  • Bohanec B, Neskovic M, Vujicic R (1993) Anther culture and androgenetic plant regeneration in buckwheat (Fagopyrum esculentum Moench). Plant Cell, Tissue Organ Cult 35:259–266

    Article  Google Scholar 

  • Bourgin JP, Nitsch JP (1967) Obtention de Nicotiana haploides a partir d’etamines cultivees in vitro. Ann Physiol Veg 9:377–382

    Google Scholar 

  • Bugara AM (1986) Embryoidogenesis in anther culture of Salvia sclarea. Fiziologiia I Biokhimiia Kulturnykh Rastenii. Physiol Biochem Cultiv Plants 18:381–386

    Google Scholar 

  • Chand S, Basu P (1998) Embryogenesis and plant regeneration from callus cultures derived from unpollinated ovaries of Hyoscyamus muticus L. Plant Cell Rep 17:302–305

    Article  CAS  Google Scholar 

  • Chand S, Sahrawat AK (2007) Embryogenesis and plant regeneration from unpollinated ovary culture of Psoralea corylifolia. Biol Plant 51:223–228

    Article  CAS  Google Scholar 

  • Chaturvedi R, Razdan MK, Bhojwani SS (2003) Production of haploids of neem (Azadirachta indica A. Juss.) by anther culture. Plant Cell Rep 21:531–537

    PubMed  CAS  Google Scholar 

  • Chen JF, Cui L, Malik AA, Mbira KG (2011) In vitro haploid and dihaploid production via unfertilized ovule culture. Plant Cell, Tissue Organ Cult 104:311–319

    Article  Google Scholar 

  • Chu CC (1978) The N6 medium and its applications to anther culture of cereal crops. In Proceedings of symposium on plant tissue culture, Science Press, Beijing, 43–50

    Google Scholar 

  • Corduan G, Spix S (1975) Haploid callus and regeneration of plants from anthers of Digitalis purpurea L. Planta 124:1–11

    Article  Google Scholar 

  • Dieu P, Dunwell JM (1988) Anther culture with different genotypes of opium poppy (Papaver somniferum L.): effect of cold treatment. Plant Cell, Tissue Organ Cult 12:263–271

    Article  Google Scholar 

  • Doi H, Takahashi R, Hikage T, Takahata Y (2010) Embryogenesis and doubled haploid production from anther culture in gentian (Gentiana triflora). Plant Cell, Tissue Organ Cult 102:27–33

    Google Scholar 

  • Doi H, Yokoi S, Hikage T, Nishihara M, Tsutsumi K, Takahata Y (2011) Gynogenesis in gentians (Gentiana triflora, G. scabra): production of haploids and doubled haploids. Plant Cell Rep 30:1099–1106

    Google Scholar 

  • Du LG, Hou YH, Chang WC, Li AS, Fu ZM, Shao QQ, Chen ZG, Yang ZT (1987) In vitro pollen plant induction and embryoid clone establishment of Panax ginseng. Sci Sin B 30:941–945

    PubMed  CAS  Google Scholar 

  • Dunwell JM (2010) Haploids in flowering plants: origins and exploitation. Plant Biotechnol J 8:377–424

    Article  PubMed  CAS  Google Scholar 

  • Ferrie AMR (2007) Doubled haploid production in nutraceutical species: a review. Euphytica 158:347–357

    Article  Google Scholar 

  • Ferrie AMR (2009) Current status of doubled haploids in medicinal plants. In: Touraev A, Forster BP, Jain SM (eds) Advances in haploid production in higher plants. Springer Science + Business Media B.V, Dordrecht, pp 209–217

    Google Scholar 

  • Ferrie AMR, Bethune T, Kernan Z (2005) An overview of preliminary studies on the development of doubled haploid protocols for nutraceutical species. Acta Physiol Plant 27:735–741

    Article  Google Scholar 

  • Ferrie AMR, Bethune T, Mykytyshyn M (2011) Microspore embryogenesis in the Apiaceae. Plant Cell, Tissue Organ Cult 104:399–406

    Article  Google Scholar 

  • Ferrie AMR, Caswell KL (2011) Isolated microspore culture techniques and recent progress for haploid and doubled haploid plant production. Plant Cell, Tissue Organ Cult 104:301–309

    Article  Google Scholar 

  • Ferrie AMR, MÅ‘llers C (2011) Haploids and doubled haploids in Brassica spp. for genetic and genomic research. Plant Cell, Tissue Organ Cult 104:375–386

    Article  Google Scholar 

  • Ferrie AMR, Palmer CE, Keller WA (1995) Haploid embryogenesis. In: Thorpe TA (ed) In vitro embryogenesis in plants. Kluwer Academic Publishers, Dordrecht, pp 309–344

    Chapter  Google Scholar 

  • Flachsland E, Mroginski L, Daviña J (1996) Regeneration of plants from anthers of Stevia rebaudiana Bertoni (Compositae) cultivated in vitro. Biocell 20:87–90

    CAS  Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158

    Article  PubMed  CAS  Google Scholar 

  • Gharyal PK, Rashid A, Maheshwari SC (1983a) Production of haploid plantlets in anther cultures of Albizzia lebbeck L. Plant Cell Rep 2:308–309

    Article  CAS  Google Scholar 

  • Gharyal PK, Rashid A, Maheshwari SC (1983b) Androgenic response from cultured anthers of a leguminous tree, Cassia siamea Lam. Protoplasma 118:91–93

    Article  Google Scholar 

  • George L (1985) Anther culture of Catharanthus roseus L.—development of pollen embryoids. Curr Sci 54:641–642

    Google Scholar 

  • Germanà MA (2011a) Anther culture for haploid and doubled haploid production. Plant Cell, Tissue Organ Cult 104:283–300

    Article  Google Scholar 

  • Germanà MA (2011b) Gametic embryogenesis and haploid technology as valuable support to plant breeding. Plant Cell Rep 30:839–857

    Article  PubMed  Google Scholar 

  • Guha S, Maheshwari SC (1964) In vitro production of embryos from anthers of Datura. Nature 204:497

    Article  Google Scholar 

  • Hatano K, Shoyama Y, Nishioka I (1987) Somatic embryogenesis and plant regeneration from the anther of Aconitum carmichaeli Debx. Plant Cell Rep 6:446–448

    CAS  Google Scholar 

  • Iqbal MCM, Wijesekara KB (2007) A brief temperature pulse enhances the competency of microspores for androgenesis in Datura metel. Plant Cell, Tissue Organ Cult 89:141–149

    Article  Google Scholar 

  • Kernan Z, Ferrie AMR (2006) Microspore embryogenesis and the development of a double haploidy protocol for cow cockle (Saponaria vaccaria). Plant Cell Rep 25:274–280

    Article  PubMed  CAS  Google Scholar 

  • Laurain D, Trémouillaux-Guiller J, Chénieux J (1993) Embryogenesis from microspores of Ginkgo biloba L., A medicinal woody species. Plant Cell Rep 12:501–505

    Google Scholar 

  • Lichter R (1982) Induction of haploid plants from isolated pollen of Brassica napus. Z Pflanzenphysiol 105:427–434

    Google Scholar 

  • Linsmaier EM, Skoog F (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol Plant 18:100–127

    Article  CAS  Google Scholar 

  • Maluszynski M, Kasha KJ, Forster BP, Szarejko I (2003) Doubled haploid production in crop plants: a manual. Kluwer Academic Publishers, Dordrecht

    Google Scholar 

  • Martinez LD, de Halac IN (1995) Organogenesis of anther-derived calluses in long-term cultures of Oenothera hookeri de Vries. Plant Cell, Tissue Organ Cult 42:91–96

    Article  Google Scholar 

  • Matsubara S, Dohya N, Murakami K (1995) Callus formation and regeneration of adventitious embryos from carrot, fennel and mitsuba microspores by anther and isolated microspore cultures. Acta Hortic 392:129–137

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Nitsch JP, Nitsch C (1969) Haploid plants from pollen grains. Science 163:85–87

    Article  PubMed  CAS  Google Scholar 

  • Nomizu T, Niimi Y, Han D-S (2004) Haploid plant regeneration via embryogenesis from anther cultures of Hepatica nobilis. Plant Cell, Tissue Organ Cult 79:307–313

    Article  Google Scholar 

  • Pathirana R, Frew T, Hedderley D, Timmerman-Vaughan G, Morgan E (2011) Haploid and doubled haploid plants from developing male and female gametes of Gentiana triflora. Plant Cell Rep 30:1055–1065

    Article  PubMed  CAS  Google Scholar 

  • Pérez-Bermúdez P, Cornejo MJ, Segura J (1985) Pollen plant formation from anther cultures of Digitalis obscura L. Plant Cell, Tissue Organ Cult 5:63–68

    Article  Google Scholar 

  • Prakash DVSSR, Chand S (1999) In vitro response from cultured anthers of Boswellia serrata Roxb. (Burseraceae). In: Plant tissue culture and biotechnology: emerging trends. Universities Press, Hyderbad, pp 226–231

    Google Scholar 

  • Raghavan V, Nagmani R (1989) Cytokinin effects on pollen embryogenesis in cultured anthers of Hyoscyamus niger. Can J Bot 67:247–257

    Article  CAS  Google Scholar 

  • Rines HW, Riera-Lizarazu O, Maquieira SB, Phillips RL (1996) Wide crosses for haploids. In: Scoles G, Rossnagel B (eds) In: Proceedings of V international oat conference and VII international barley genetics symposium. University of Saskatchewan, Saskatoon, pp 213–222

    Google Scholar 

  • Samsudden K, Babu KN, Divakaran M, Ravindran PN (2000) Plant regeneration from anther derived callus cultures of ginger (Zingiber officinale Rosc.). J Hortic Sci Biotechnol 75:447–450

    Google Scholar 

  • Schulte J, Büter B, Schaffner W, Berger K (1996) Gametic embryogenesis in Hypericum spp. In: Pank F (ed) International symposium on breeding research on medicinal and aromatic plants, Quedlinburg, pp 307–310

    Google Scholar 

  • Sestili S, Ficcadenti N (1996) Irradiated pollen for haploid production. In: Jain SM, Sopory SK, Veilleux RE (eds) In vitro haploid production in higher plants, vol 1 Kluwer Academic Publishers, Dordrecht, pp 263–274

    Google Scholar 

  • Shao Q, Li A (1986) Somatic embryogenesis and plant regeneration from anther culture of Panax quinquifolius (Ginseng). Int Plant Biotechnol Netw 6:2

    Google Scholar 

  • Shon T-K, Yoshida T (1997) Induction of haploid plantlets by anther culture of Bupleurum falcatum L. Jpn J Crop Sci 66:137–138

    Article  Google Scholar 

  • Shon T-K, Kim S-K, Acquah D, Lee S-C (2004) Haploid plantlet production through somatic embryogenesis in anther-derived callus of Bupleurum falcatum. Plant Prod Sci 7:204–211

    Article  Google Scholar 

  • Singh MN, Konar RN (1981) In vitro induction of haploid roots and shoots from female gametophyte of Ephedra foliate Boiss. Beltr Biol Pflanzen 55:169–177

    Google Scholar 

  • Smýkalová I, Å mirous P Jr, KuboÅ¡iová M, Gasmanová N, Griga M (2009) Doubled haploid production via anther culture in annual, winter type of caraway (Carum carvi L.). Acta Physiol Plant 31:21–31

    Article  Google Scholar 

  • Suh SK, Park HG (1986) Studies on the anther culture of garlic (Allium sativum L.). J Korean Soc Hortic Sci 27:89–95

    Google Scholar 

  • Takahata Y, Fukuoka H, Wakui K (2005) Utilization of microspore-derived embryos. In: Palmer CE, Keller WA, Kasha KJ (eds) Biotechnology in agriculture and forestry: haploids in crop improvement II. 56, pp 153–169

    Google Scholar 

  • Theiler-Hedtrich R, Hunter CS (1996) Microspore culture in chicory (Cichorium intybus L.). In: Jain SM, Sopory SK, Veilleux RE (eds) In vitro haploid production in higher plants, vol 3, Kluwer Academic Publishers, Dordrecht, pp 99–113

    Google Scholar 

  • Touraev A, Heberle-Bors E (1999) Microspore embryogenesis and in vitro pollen maturation in tobacco. In: Hall RD (eds) Methods in molecular biology: Plant cell culture protocols, vol 111. Humana Press, Totowa, pp 281–291

    Google Scholar 

  • Ulrich A, Furton WH, Downey RK (1984) Biotechnology and rapeseed breeding: some economic considerations. Sci. Counc. Can. Rep, Ottawa

    Google Scholar 

  • Vishnoi A, Babbar SB, Gupta SC (1979) Induction of androgenesis in anther cultures of Withania somnifera. Z Pflanzen 94:169–171

    CAS  Google Scholar 

  • White PR (1943) Handbook of plant tissue culture. Jacques Cattell Press, Lancaster, 277 pp

    Book  Google Scholar 

  • Zenkteler M (1971) In vitro production of haploid plants from pollen grains of Atropa belladonna L. Experientia 27:1087

    Article  Google Scholar 

  • Zhao F-C, Nilanthi D, Yang Y-H, Wu H (2006) Anther culture and haploid plant regeneration in purple coneflower (Echinacea purpurea L.). Plant Cell Rep 86:55–62

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Alison M. R. Ferrie .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2013 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Ferrie, A.M.R. (2013). Advances in Microspore Culture Technology: A Biotechnological Tool for the Improvement of Medicinal Plants. In: Chandra, S., LATA, H., Varma, A. (eds) Biotechnology for Medicinal Plants. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-29974-2_8

Download citation

Publish with us

Policies and ethics