Abstract
The breeding companies and laboratories involved in this article cover a wide range of crops grown in the temperate climate zone: small grain cereals, oilseed crops, forage crops, turf, vegetables and potato. Speed and efficiency are becoming increasingly important in variety breeding and doubled haploids (DH) and genetic markers are important biotechnological tools to accelerate materials to market. Collaborative research between universities, research institutions and breeding companies has resulted in the routine use of DH technology and molecular markers in practical breeding of barley, wheat and rapeseed. DH populations have been established not only for barley, wheat and rapeseed, but for rye, oat and triticale, where DH technology is less developed. A driver here is the value of the crop e.g. although wheat is less responsive to DH production the value of the end product makes the effort worthwhile. Simple and rapid DNA extraction methods used in high-throughput marker assisted selection (MAS) systems are essential for routine use of markers. MAS is used both to monitor the presence of genes of interest and also to monitor the genetic background. DH technology in forage, turf and vegetables is still in progress and the practical use of markers in all crops is limited by access to trait linked markers. Collaboration and technology transfer with universities, research institutions and breeding companies is essential for the improvement of both DH protocols in recalcitrant crops and marker technology in all crops.
Similar content being viewed by others
References
Adamus A, Michalik B (2003) Anther culture of carrot (Daucus carota L.). Folia horticulturate 15:49–58
Andersen SB (2003) Double haploid induction in ryegrass and other grasses. In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (eds) Doubled haploid production in crop plants—a manual. Kluwer Academic Publishers, Dordrecht/Boston/London, pp 179–183
Andersen JR, Lübberstedt T (2003) Functional markers in plants. Trends Plant Sci 8:554–560
Bertholdsson, N-O, Tuvesson S (2005) Possibilities to use marker assisted selection to improve allelopathic activity in cereals. COST 860 SUSVAR ‘Organic Breeding strategies and the Use of Molecular markers’ 17–19 January 2005, Driebergen, The Netherlands
Custers JBM (2003) Microspore culture in rapeseed (Brassica napus L.). In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (eds) Doubled haploid production in crop plants—a manual. Kluwer Academic Publishers, Dordrecht/Boston/London, pp 185–193
Dayteg C, Tuvesson S, Öhlund R, von Post L, Nilsson M, Lasson G (1999) Automation of molecular markers at SvalÖf Weibull AB. Gatersleben Research Conference. Plant Genomics. Structural, Functional and Applied Aspects. IPK Gatersleben, Germany, June 17–21, 1999.
De Vienne D (2003) Molecular markers in plant genetics and biotechnology. Science Publishers, Inc., Enfield (NH), USA, Plymouth, UK
Foisset N, Trahan C, Cheung WY, Hubert N, Tuvesson S, Happstadius I, Landry BS (1998) Study of a major resistance gene to race PG2 of the pathogen causing blackleg disease: use of a linked co-dominant PCR marker to determine the gene effect and the genome origin. Eleventh Crucifer Genetics Workshop. ISHS Symposium on Brassicas. Montreal (Canada), October 3–7, 1998
Ford CM, Paltridge NG, Rathjen JP, Moritz RL, Simpson RI, Symonds RH (1998) Rapid and informative assays for Yd2, the barley yellow dwarf virus resistance gene, based on the nucleotide sequence of a closely linked gene. Mol breeding 4:23–31
Forster BP, Thomas WTB (2004) Doubled haploids in genetics and plant breeding. Plant Breed Rev 25:57–88
Graner A, Streng S, Kellermann A, Schiemann A, Bauer E, Waugh R, Pellio B, Ordon F (1999) Molecular mapping and genetic fine-structure of the rym5 locus encoding resistance to different strains of the Barley Yellow Mosaic Virus Complex. Theor Appl Genet 98:285–290
Hansen M (2000) ABA treatment and desiccation of microspore-derived embryos of cabbage (Brassica oleracea ssp. capitata L) improves plant development. J Plant Physiol 156:164–167
Hansen M (2003) Protocol for microspore culture in Brassica. In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (eds) Doubled haploid production in crop plants—a manual. Kluwer Academic Publishers, Dordrecht/Boston/London, pp 215–222
Horvath DP, Dahleen LS, J-A Stebbing, Penner G (1995) A co-dominant PCR-based marker for assisted selection of durable stem rust resistance in barley. Crop Sci 35:1445–1450
Ivandic V, Walther U, Graner A (1998) Molecular mapping of a new gene in wild barley conferring complete resistance to leaf rust (Puccinia hordei Otth). Theor Appl Genet 97:1235–1239
Immonen S, Robinson J (2000) Stress treatments and ficoll for improving green plant regeneration in triticale anther culture. Plant Sci 150:77–84
Immonen S, Tenhola-Roininen T (2003) Protocol for rye anther culture. In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (eds) Doubled haploid production in crop plants—a manual. Kluwer Academic Publishers, Dordrecht/Boston/London, pp. 141–149
Islam MR, Kintzios S, Fischbeck G (1992) Anther culture responsiveness of Hordeum spontaneum derived spring barley lines and a genetic analysis of plant regeneration. Plant Cell Tissue Organ Cult 29:235–239
Jacquard C, Wojnarowiez G, Clément C (2003) Anther culture in barley. In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (eds) Doubled haploid production in crop plants—a manual. Kluwer Academic Publishers, Dordrecht/Boston/London, pp 21–27
Kilian A, Steffenson BJ, Saghai Maroof MA, Kleinhofs A (1994) RFLP markers linked to the durable stem rust resistance gene Rpg1 in barley. Molecular Plant–Microbe Interactions 7(2):298–301
Kiviharju E, Moisander S, Laurila J (2005) Improved green plant regeneration rates from oat anther culture and the agronomic performance of some DH lines. Plant Cell Tissue Organ Cult 81:1–9
Ma C, Fu T, Bengtsson L, Gertsson B, Dayteg C, Tuvesson S (2003) Genetic diversity of Chinese and Swedish Brassica napus cultivars based on Inter-SSR PCR markers and its relationship to hybrid performance. J Swedish Seed Assoc 2:67–77
Manninen O, Tanhuanpää P, Tenhola-Roininen T, Kiviharju E (2004) Doubled haploids and genetic mapping in barley, rye and oat. In: Vollmann J, Grausgruber H, Ruckerbauer R (eds) Genetic variation for plant breeding. Publisher BOKU – University of Natural Resources and Applied Life Sciences, Vienna, Austria, p 30
Mano Y, Sayed-Tabatabaei BE, Graner A, Blake T, Takaiwa F, Oka S, Komatsuda T (1999) Map construction of sequence-tagged sites (STSs) in barley (Hordeum vulgare L.). Theor Appl Genet 98:937–946
Mohler V, Jahoor A (1996) Allele-specific amplification of polymorphic sites for the detection of powdery mildew resistance loci in cereals. Theor Appl Genet 93:1078–1082
Nishio T, Kusaba M, Watanabe M, Hinata K (1996) Registration of S alleles in Brassica campestris L by the restriction fragment of SLGs. Theor Appl Genet 92:388–394
Olesen A, Andersen SB, Due IK (1988) Anther culture response in perennial ryegrass (Lolium perenne L.). Plant Breed 101:60–65
Paltridge NG, Collins NC, Bendahmane A, Symons RH (1998) Development of YLM, a codominant marker closely linked to the Yd2 gene for resistance to barley yellow dwarf disease. Theor Appl Genet 96:1170–1177
Ramsay L, Macaulay M, degli Ivanissevich S, MacLean K, Cardle L, Fuller J, Edwards K, Tuvesson S, Morgante M, Massari A, Maesti E, Marmiroli N, Sjakste T, Ganal M, Powell W, Waugh R (2000) A simple sequence repeat - based linkage map of barley. Genetics 156:1997–2005
Rokka V-M, Pietilä L, Pehu E (1996) Enhanced production of dihaploid lines via anther culture of tetraploid potato (Solanum tuberosum L. ssp. tuberosum) clones American Potato Journal 73:1–12
Swanston JS, Thomas WTB, Powell W, Young GR, Lawrence PE, Ramsay L, Waugh R, (1999) Using molecular markers to determine barleys most suitable for malt whisky distilling. Mol Breeding 5:103–109
Tenhola T, Tanhuanpää P, Immonen S (2001) Doubled haploids and DNA markers in rye breeding. In: Bohanec B (ed) Biotechnological approaches for utilisation of gametic cells. Cost Action 824, Belgium, pp. 73–77
Tuvesson S, v Post L, Öhlund R, Hagberg P, Graner A, Svitashev S, Schehr M, Elovsson R (1998) Molecular breeding for the BaMMV/BaYMV resistance gene ym4 in winter barley. Plant Breed 117:19–22
Tuvesson S, v Post R, Ljungberg A (2003) Wheat anther culture. In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (eds) Doubled haploid production in crop plants—a manual. Kluwer Academic Publishers, Dordrecht/Boston/London, pp 71–76
Von Post R, v Post L, Dayteg C, Nilsson M, Forster BP, Tuvesson S (2003) A high-throughput DNA extraction method for barley seed. Euphytica 130(2):255–260
Werner K, Friedt W, Ordon F (2005) Strategies for pyramiding resistance genes against the barley yellow mosaic virus complex (BaMMV, BaYMV, BaYMV-2). Mol. Breeding 16:45–55
Åhman I, Tuvesson S, Johansson M (2000) Does the alkaloid gramine confer resistance in barley to the aphid Rhopalosiphum padi ?. J Chem Ecol 26(1):233–255
Acknowledgements
The Swedish Farmer’s Foundation for Agricultural Research and FORMAS are acknowledged for financial support (S. Tuvesson and C. Dayteg). O. Manninen, P. Tanhuanpää, T. Tenhola-Roininen and E. Kiviharju acknowledge the Ministry of Agriculture and Forestry of Finland and J. Weyen, J. Schondelmaier and J. Förster acknowledge EU project TEGERM for financial support.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Tuvesson, S., Dayteg, C., Hagberg, P. et al. Molecular markers and doubled haploids in European plant breeding programmes. Euphytica 158, 305–312 (2007). https://doi.org/10.1007/s10681-006-9239-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10681-006-9239-8