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Laser Microdissection as a Useful Tool to Study Gene Expression in Plant and Fungal Partners in AM Symbiosis

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Arbuscular Mycorrhizal Fungi

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2146))

Abstract

Laser microdissection (LMD) technology has been widely applied to plant tissues, offering novel information on the role of different cell-type populations during plant–microbe interactions. In this chapter, protocols to apply the LMD approach to study plant and fungal transcript profiles in different cell-type populations from arbuscular mycorrhizal (AM) roots are described in detail, starting from the biological material preparation to gene expression analyses by RT-PCR and RT-qPCR.

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Acknowledgments

The authors thank Jorge Gómez-Ariza and Marco Giovannetti for their help to optimize the described protocols and the helpful discussion during the experiments.

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Authors and Affiliations

  1. National Research Council—Institute for Sustainable Plant Protection (CNR-IPSP), Turin Unit, Torino, Italy

    Raffaella Balestrini

  2. University of Turin—Department of Life Sciences and Systems Biology (UNITO-DBIOS), Torino, Italy

    Valentina Fiorilli

Authors
  1. Raffaella Balestrini
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  2. Valentina Fiorilli
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Corresponding authors

Correspondence to Raffaella Balestrini or Valentina Fiorilli .

Editor information

Editors and Affiliations

  1. Department of Soil Microbiology and Symbiotic Systems, Estación Experimental del Zaidín, CSIC, Granada, Spain

    Nuria Ferrol

  2. Dipartimento di Scienze della Vita e Biologia dei Sistemi, Università di Torino, Torino, Italy

    Luisa Lanfranco

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Balestrini, R., Fiorilli, V. (2020). Laser Microdissection as a Useful Tool to Study Gene Expression in Plant and Fungal Partners in AM Symbiosis. In: Ferrol, N., Lanfranco, L. (eds) Arbuscular Mycorrhizal Fungi. Methods in Molecular Biology, vol 2146. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0603-2_13

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  • DOI: https://doi.org/10.1007/978-1-0716-0603-2_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0602-5

  • Online ISBN: 978-1-0716-0603-2

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