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Natural and Modified Promoters for Tailored Metabolic Engineering of the Yeast Saccharomyces cerevisiae

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Yeast Metabolic Engineering

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1152))

Abstract

The ease of highly sophisticated genetic manipulations in the yeast Saccharomyces cerevisiae has initiated numerous initiatives towards development of metabolically engineered strains for novel applications beyond its traditional use in brewing, baking, and wine making. In fact, baker’s yeast has become a key cell factory for the production of various bulk and fine chemicals. Successful metabolic engineering requires fine-tuned adjustments of metabolic fluxes and coordination of multiple pathways within the cell. This has mostly been achieved by controlling gene expression at the transcriptional level, i.e., by using promoters with appropriate strengths and regulatory properties. Here we present an overview of natural and modified promoters, which have been used in metabolic pathway engineering of S. cerevisiae. Recent developments in creating promoters with tailor-made properties are also discussed.

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Authors and Affiliations

  1. Molecular Systems Biology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands

    Georg Hubmann

  2. Laboratory of Molecular Cell Biology, Institute of Botany and Microbiology, KU Leuven, Leuven-Heverlee, Flanders, Belgium

    Georg Hubmann & Johan M. Thevelein

  3. Department of Molecular Microbiology, VIB, Kasteelpark Arenberg 31, B-3001, Leuven-Heverlee, Flanders, Belgium

    Georg Hubmann & Johan M. Thevelein

  4. School of Engineering and Science, Jacobs University gGmbH, Campus Ring 1, 28759, Bremen, Germany

    Elke Nevoigt

Authors
  1. Georg Hubmann
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  2. Johan M. Thevelein
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  3. Elke Nevoigt
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Corresponding author

Correspondence to Elke Nevoigt .

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Editors and Affiliations

  1. Industrial Biotechnology, Chalmers University of Technology, Gothenburg, Sweden

    Valeria Mapelli

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Hubmann, G., Thevelein, J.M., Nevoigt, E. (2014). Natural and Modified Promoters for Tailored Metabolic Engineering of the Yeast Saccharomyces cerevisiae . In: Mapelli, V. (eds) Yeast Metabolic Engineering. Methods in Molecular Biology, vol 1152. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0563-8_2

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  • DOI: https://doi.org/10.1007/978-1-4939-0563-8_2

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