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Systems for applied gene control in Saccharomyces cerevisiae

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Abstract

Saccharomyces cerevisiae is frequently used in biotechnology, including fermentative processes in food production, heterologous protein production and high throughput developments for biomedicine. Accurate expression of selected genes is essential for all these areas. Systems that can be regulated are particularly useful because they allow controlling the timing and levels of gene expression. We examine here new expression systems that have been described, including improvements of classical ones and new strategies of artificial gene control that have been applied in functional genomics.

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Acknowledgments

This work was supported by the Spanish Ministry for Education and Science (FIT-010000-2003-110 and CIT-010000-2005-32), by the Andalusian Government (CVI271) and by Biomedal, SL. We thank Ángel Cebolla for his intellectual inputs and Miguel Arévalo-Rodríguez for revising the manuscript.

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Authors and Affiliations

  1. Departamento de Genética, Universidad de Sevilla, Avda. Reina Mercedes 6, 41012, Seville, Spain

    Douglas Maya, María José Quintero, María de la Cruz Muñoz-Centeno & Sebastián Chávez

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  1. Douglas Maya
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  2. María José Quintero
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  3. María de la Cruz Muñoz-Centeno
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  4. Sebastián Chávez
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Correspondence to Sebastián Chávez.

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Maya, D., Quintero, M.J., de la Cruz Muñoz-Centeno, M. et al. Systems for applied gene control in Saccharomyces cerevisiae . Biotechnol Lett 30, 979–987 (2008). https://doi.org/10.1007/s10529-008-9647-z

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  • DOI: https://doi.org/10.1007/s10529-008-9647-z

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