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PD-L1 Detection on Circulating Melanoma Cells

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Melanoma

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2265))

Abstract

The advent of personalized medicines targeting cell signaling pathways has radically improved melanoma patient outcomes. More recently, immune-modulating therapies disrupting the PD-1/PD-L1 axis have become a powerful tool in the treatment of a range of melanoma, showing a profound improvement in the overall survival outcomes. However, immune checkpoint inhibitors (ICIs) are associated with considerable toxicities and appear to only be efficacious in a subset of melanoma patients. Therefore, there is an urgent need to identify biomarkers that can determine if patients will or will not respond to ICI therapy. Here, we describe an optimized method for analyzing PD-L1 expression on circulating melanoma cells following immunomagnetic enrichment from patient blood samples.

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Acknowledgments

This work was supported by the Cancer Institute New South Wales through the Centre for Oncology Education and Research Translation (CONCERT, grant ID: 13/TRC/1-01). Human ethics approval, HREC/13/LPOOL/158, was obtained and managed by the CONCERT Biobank.

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Authors and Affiliations

  1. Centre for Circulating Tumour Cell Diagnostics & Research at the Ingham Institute for Applied Medical Research, Liverpool, NSW, Australia

    Joseph W. Po, Yafeng Ma, Daniel Brungs, Vinay Murthy, Paul de Souza & Therese M. Becker

  2. Centre for Oncology Education and Research Translation (CONCERT), Liverpool, NSW, Australia

    Joseph W. Po, Yafeng Ma, Daniel Brungs, Adam Cooper, Vinay Murthy & Therese M. Becker

  3. Western Sydney University, School of Medicine, Campbelltown, NSW, Australia

    Joseph W. Po, Adam Cooper, Erin Saricilar, Vinay Murthy, Paul de Souza & Therese M. Becker

  4. University of New South Wales, School of Medicine, Kensington, NSW, Australia

    Yafeng Ma, Erin Saricilar, Paul de Souza & Therese M. Becker

  5. Liverpool Hospital, Liverpool, NSW, Australia

    Bavanthi Balakrishnar, Farhad Azimi, Adam Cooper, Erin Saricilar, Vinay Murthy & Paul de Souza

  6. University of Wollongong, School of Medicine, Wollongong, NSW, Australia

    Daniel Brungs & Paul de Souza

  7. Illawarra Cancer Centre, Wollongong Hospital, Wollongong, NSW, Australia

    Daniel Brungs

  8. University of Sydney, Camperdown, NSW, Australia

    Erin Saricilar

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  1. Joseph W. Po
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  2. Yafeng Ma
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  3. Bavanthi Balakrishnar
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  4. Daniel Brungs
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  7. Erin Saricilar
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  8. Vinay Murthy
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  9. Paul de Souza
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  10. Therese M. Becker
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Correspondence to Joseph W. Po .

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Editors and Affiliations

  1. Department of Biology, Hampden-Sydney College, Hampden-Sydney, VA, USA

    Kristian M. Hargadon

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Po, J.W. et al. (2021). PD-L1 Detection on Circulating Melanoma Cells. In: Hargadon, K.M. (eds) Melanoma. Methods in Molecular Biology, vol 2265. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1205-7_17

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  • DOI: https://doi.org/10.1007/978-1-0716-1205-7_17

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1204-0

  • Online ISBN: 978-1-0716-1205-7

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