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Synthesis of Magnetic Fe3O4 Nanorings for BSA Protein Adsorption

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Clays and Clay Minerals

Abstract

The adsorption of bovine serum albumin (BSA) protein is important for protein research but remains a great challenge. Here, the hydrothermal method and calcination in a hydrogen-argon mixed atmosphere were used to obtain ordered mono-crystalline magnetic Fe3O4 nanorings with controllable size and uniform structure, which were applied to the adsorption of BSA protein. The results showed that the magnetic Fe3O4 nanorings prepared have the advantages of good crystallinity, controllable morphology, and excellent magnetic response. The Fe3O4 nanorings had an outer diameter of ~160–180 nm, inner diameter of ~80–110 nm, and height of 70 to ~110 nm. The amount of BSA adsorbed by magnetic Fe3O4 nanorings increased with increasing protein concentration; when the concentration of BSA was ~2.75 mg·mL-1 the adsorption amount approached saturation. When the pH value of the solution was ~5, the Fe3O4 nanorings absorption of BSA was greatest. With increasing adsorption time, the amount adsorbed increased gradually. Adsorption equilibrium was reached after 3 h. According to the formula, the saturated adsorption capacity of BSA per gram of magnetic Fe3O4 nanorings was 325.2 mg.

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Acknowledgements

This work was supported by the Scientific Research Fund of Sichuan Provincial Education Department (17ZA0401), Fundamental Science on Nuclear Wastes and Environmental Safety Laboratory (15kffk05), Sichuan Science and Technology Program (2018JY0315), China Postdoctoral Science Foundation (2018M633411) and Research Support Program for Longshan Academic Talents (18LZX673), National Natural Science Foundation of China (41872039 and 41831285), and the One-Thousand-Talents Scheme in Sichuan Province, Sichuan Science and Technology Program(2018JY0462).

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Authors and Affiliations

  1. State Key Laboratory for Environment-Friendly Energy Materials, Southwest University of Science and Technology, Mianyang, 621010, People’s Republic of China

    Xiaonan Liu

  2. Institute of Chemical Materials, China Academy of Engineering Physics (CAEP), Mianyang, 621900, People’s Republic of China

    Xiaonan Liu & Jinshan Li

  3. National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei, 230026, People’s Republic of China

    Youkui Zhang

  4. Key Laboratory of Solid Waste Treatment and Resource Recycle, Ministry of Education, Southwest University of Science and Technology, Mianyang, 621010, People’s Republic of China

    Liang Bian

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  1. Xiaonan Liu
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  2. Youkui Zhang
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  4. Jinshan Li
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Correspondence to Xiaonan Liu or Jinshan Li.

Additional information

This paper was originally presented during the World Forum on Industrial Minerals, held in Qing Yang, China, October 2018.

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Liu, X., Zhang, Y., Bian, L. et al. Synthesis of Magnetic Fe3O4 Nanorings for BSA Protein Adsorption. Clays Clay Miner. 67, 275–282 (2019). https://doi.org/10.1007/s42860-019-00026-z

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  • DOI: https://doi.org/10.1007/s42860-019-00026-z

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