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The metabolic constituent and rooting-related enzymes responses of marigold explants to hydrogen gas during adventitious root development

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Abstract

Recent evidence suggests that hydrogen gas is involved in multiple physiological process in plants. However, the physiological mechanism of hydrogen gas on adventitious rooting is largely unknown. In this report, marigold (Tagetes erecta L.) was used to determine the effect of hydrogen gas on physiological changes during adventitious root development. The results showed that 50% hydrogen-rich water significantly increased root number and length of marigold explants. Hydrogen-rich water treatment provoked a significant reduction of stomatal aperture which might be associated with the increase of relative water content. Hydrogen-rich water decreased electrolyte leakage during adventitious rooting. The content of water-soluble carbohydrate, starch and soluble protein were higher in normal water-treated controls than in hydrogen-rich water-treated explants. Compared with the control, the application of hydrogen-rich water increased peroxidases, polyphenol oxidase and indoleacetic acid oxidase activity. Results indicated that hydrogen gas promotes adventitious root development through the increase of relative water content, metabolic constituents, rooting-related enzymes and simultaneously keeping cell membrane integrity.

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Acknowledgements

This research was supported by the National Natural Science Foundation of China (Nos. 31160398 and 31560563), the Key Project of Chinese Ministry of Education (No. 211182), the Research Fund for the Doctoral Program of Higher Education (No. 20116202120005), the Natural Science Foundation of Gansu Province, China (Nos. 1606RJZA073, 1606RJZA077, 1308RJZA179, and 1308RJZA262) and Feitian and Fuxi Excellent Talents in Gansu AgriculturalUniversity in Lanzhou, P. R. China. The authors are grateful to the editors and the anonymous reviewers for their valuable comments and help.

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  1. College of Horticulture, Gansu Agricultural University, Lanzhou, 730070, People’s Republic of China

    Yongchao Zhu & Weibiao Liao

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  1. Yongchao Zhu
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  2. Weibiao Liao
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ZY and LW designed the research. ZY conducted experiments. ZY analyzed the data. ZY and LW wrote the manuscript.

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Correspondence to Weibiao Liao.

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Zhu, Y., Liao, W. The metabolic constituent and rooting-related enzymes responses of marigold explants to hydrogen gas during adventitious root development. Theor. Exp. Plant Physiol. 29, 77–85 (2017). https://doi.org/10.1007/s40626-017-0085-y

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  • DOI: https://doi.org/10.1007/s40626-017-0085-y

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