Abstract
Cytoplasmic and salt-extracted peroxidase and IAA oxidase activities were studied in Phaseolus vulgaris hypocotyls treated with gibberellic acid (GA, 200 µM), naphthyl acetic acid (NAA, 100 µM) and distilled water control (DW). Peroxidase activity was assayed with four hydrogen donors during the initial phase of hypocotyl elongation. Though peroxidase activity showed a decreasing trend with time in all the hydrogen donors studied; considerable variation with different hydrogen donors was observed. NAA had maximum peroxidase activity as compared to DW or GA treatment. The activity showed a clear inverse correlation with hypocotyl growth. IAA oxidase activity showed a similar trend with growth as peroxidase activity. A highly significant correlation was observed between peroxidase and IAA oxidase activities and high molecular weight xyloglucan content (P<0.001). Finally, the possible role of peroxidase and IAA oxidase activities in hypocotyl elongation growth is discussed.
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References
Bagatharia S.B., Chanda S.V. 1998. Modification of cell wall polysaccharides during cell elongation in Phaseolus vulgaris hypocotyls. Acta Physiol. Plant. (in this issue).
Bottger M., Bouchet M., Gaspar R. 1978. Effect du chlorue de (2-chloroethyl)trimethyl ammonium (CCC) sur la croissance et la distribution des peroxydases due Ble. Compte Rendu Hebd des Seances de l’Acad des Sci 286:1873–1875.
Chanda S.V., Singh Y.D. 1997. Changes in peroxidase and IAA oxidase activities during wheat grain development. Plant Physiol. Biochem. 35:245–250.
Dodeema H., Telkamp G.P. 1979. Uptake of nitrate by mutant of Arabidopsis thaliana, distributed in uptake or reduction of nitrate. II Kinetics. Physiol. Plant. 45:332–338.
Dubouchet J. 1972. Some aspects of the structure and of functions of plant peroxidases. Ann. Sci. Univ. Besancon. Bot. 3:103–111.
Edelmann H., Bergfeld R., Schopfer P. 1989. Role of cell-wall biogenesis in the initiation of auxin-mediated growth in coleoptiles of Zea mays L. Planta 179:486–494.
Fry S.C. 1986. Cross-linking of matrix polymers in the growing cell wall of angiosperms. Annu. Rev. Plant Physiol. 37:165–186.
Gibson D.M., Liu E.H. 1978. Substrate specificities of peroxidase isozymes in the developing pea seedling. Ann Bot 42:1075–1083.
Gordon S.A., Weber R.P. 1951. Colorimetric estimation of indoleacetic acid. Plant Physiol. 26:192–195.
Jupe S.C., Scott I.M. 1989. The slender phenotype of pea: stem growth, peroxidase levels and ethylene responses. Physiol. Plant. 77:59–66.
Sembdner D.G., Liebisch H.W., Schneider G. 1980. Biosynthesis and metabolism of plant hormones. In: Encyclopedia of Plant Physiology — Hormonal Regulation of Development. I. Molecular Aspects of Plant Hormones Vol.9 Ed. Macmillan J., Springer-Verlag, Berlin pp. 281–444.
Shedletzky E., Shmuel M., Trainin T., KAlman S., Delmer D. 1992. Cell wall structure in cells adapted to growth on the cellulose- synthesis inhibitor 2,6-dichlorobenzonitrile. Plant Physiol. 100:120–130.
Terry M.E. 1985. The potential of a modified sugarcane spindle bioassay. J. Ame. Soc. Sugar Cane Tech. 5:50–54.
Thorpe T.A., Gaspar T. 1978. Changes in isoperoxidases during shoot formation in tobacco callus. In Vitro 14:522–526.
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Bagatharia, S.B., Chanda, S.V. Changes in peroxidase and IAA oxidase activities during cell elongation in Phaseolus hypocotyls. Acta Physiol Plant 20, 9–13 (1998). https://doi.org/10.1007/s11738-998-0037-x
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DOI: https://doi.org/10.1007/s11738-998-0037-x