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Organ and tissue-dependent effect of resveratrol and exercise on antioxidant defenses of old mice

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Abstract

Background

Oxidative stress has been considered one of the causes of aging. For this reason, treatments based on antioxidants or those capable of increasing endogenous antioxidant activity have been taken into consideration to delay aging or age-related disease progression.

Aim

In this paper, we determine if resveratrol and exercise have similar effect on the antioxidant capacity of different organs in old mice.

Methods

Resveratrol (6 months) and/or exercise (1.5 months) was administered to old mice. Markers of oxidative stress (lipid peroxidation and glutathione) and activities and levels of antioxidant enzymes (SOD, catalase, glutathione peroxidase, glutathione reductase and transferase and thioredoxin reductases, NADH cytochrome B5-reductase and NAD(P)H-quinone acceptor oxidoreductase) were determined by spectrophotometry and Western blotting in different organs: liver, kidney, skeletal muscle, heart and brain.

Results

Both interventions improved antioxidant activity in the major organs of the mice. This induction was accompanied by a decrease in the level of lipid peroxidation in the liver, heart and muscle of mice. Both resveratrol and exercise modulated several antioxidant activities and protein levels. However, the effect of resveratrol, exercise or their combination was organ dependent, indicating that different organs respond in different ways to the same stimulus.

Conclusions

Our data suggest that physical activity and resveratrol may be of great importance for the prevention of age-related diseases, but that their organ-dependent effect must be taken into consideration to design a better intervention.

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Acknowledgments

We thank Almudena Velazquez Dorado and Ana Sanchez Cuesta for their technical support. The group was financed by the Andalusian Government as the BIO177 Group through FEDER funds (European Commission). The research was financed by the Spanish Government Grant DEP2012-39985 (Spanish Ministry of Economy and Competitiveness). Tung Bui Thanh received a fellowship from the AECID program (Spanish Ministry of Foreing Affair). ERB, PN and GLL are also members of the Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), Instituto Carlos III.

Conflict of interest

On behalf of all authors, the corresponding author states that there is no conflict of interest.

Human and Animal Rights

All animals were maintained according to a protocol approved by the Ethical Committee of the University Pablo de Olavide of resolution 03/09 and following the international rules for animal research. This article does not contain any studies with humans performed by any of the authors.

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Authors and Affiliations

  1. School of Medicine and Pharmacy, Vietnam National University, Hanoi, Floor 5 Building Y1, 144 Xuan Thuy, Cau Giay, Hanoi, Vietnam

    Bui Thanh Tung, Hai Nguyen Thanh & Huong Le-Thi-Thu

  2. Centro Andaluz de Biología del Desarrollo, Universidad Pablo de Olavide-CSIC, CIBERER, Instituto de Salud Carlos III, Carretera de Utrera Km. 1, 41013, Seville, Spain

    Elisabet Rodriguez-Bies, Plácido Navas & Guillermo López-Lluch

  3. Departmento de Farmacología, Facultad de Farmacia, Universidad de Sevilla, 41012, Seville, Spain

    Virginia Motilva Sanchez

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  1. Bui Thanh Tung
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  2. Elisabet Rodriguez-Bies
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  3. Hai Nguyen Thanh
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  4. Huong Le-Thi-Thu
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  7. Guillermo López-Lluch
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Correspondence to Bui Thanh Tung.

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Tung, B.T., Rodriguez-Bies, E., Thanh, H.N. et al. Organ and tissue-dependent effect of resveratrol and exercise on antioxidant defenses of old mice. Aging Clin Exp Res 27, 775–783 (2015). https://doi.org/10.1007/s40520-015-0366-8

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  • DOI: https://doi.org/10.1007/s40520-015-0366-8

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