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Clonal propagation of Cephaelis ipecacuanha

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Abstract

Shoot cultures of Cephaelis ipecacuanha A. Richard were established by using shoot tips as initial explants. Multiple shoots were obtained from node segments upon culture on B5 medium supplemented with NAA-BA (0.01–3, 5 mg/l). These shoots were rooted on B5 and 1/2 MS media containing IAA or NAA, and the regenerated plants were transferred to soil and grown in a greenhouse. The emetic alkaloids of the regenerated plants, mother plants and leaves of shoot cultures were analyzed by TLC and HPLC. Seven months of growth under greenhouse condition, the contents of the emetic alkaloids in the regenerated plants were comparable to those of the mother plants.

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Abbreviations

B5:

Gamborg B5 (1968) medium

MS:

Murashige-Skoog (1962) medium

1/2 MS:

a half strength MS medium

NAA:

1-naphthaleneacetic acid

IAA:

indole-3-acetic acid

Kin:

kinetin

BA:

6-benzylaminopurine

TLC:

thin layer chromatography

HPLC:

high performance liquid chromatography

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Authors and Affiliations

  1. Tsukuba Medicinal Plant Research Station, National Institute of Hygienic Sciences, 1 Hachimandai, Tsukuba, 305, Ibaraki, Japan

    Kayo Ideda, Motoyoshi Satake & Koichiro Shimomura

  2. Department of Hospital Pharmacy, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashi-ku, 812, Fukuoka, Japan

    Daisuke Teshima & Toshinobu Aoyama

Authors
  1. Kayo Ideda
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  2. Daisuke Teshima
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  3. Toshinobu Aoyama
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  4. Motoyoshi Satake
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  5. Koichiro Shimomura
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Communicated by F. Constabel

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Ideda, K., Teshima, D., Aoyama, T. et al. Clonal propagation of Cephaelis ipecacuanha . Plant Cell Reports 7, 288–291 (1988). https://doi.org/10.1007/BF00272545

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  • DOI: https://doi.org/10.1007/BF00272545

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