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Production of emetic alkaloid by in vitro culture of cephaelis ipecacuanha A. Richard

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Abstract

Callus and adventitious roots were induced on leaf segments from shoot culture of Cephaelis ipecacuanha A. Richard on Murashige-Skoog medium containing 2,4-dichlorophenoxyacetic acid, indole-3-acetic acid, 1-naphthaleneacetic acid and kinetin. The contents of emetic alkaloids in calli, roots and root suspension cultures were quantified by HPLC. Roots cultured in solid and liquid Murashige-Skoog media yielded emetine and cephaeline. The amount of the two alkaloids in the root suspension culture was very similar to that of roots from ipecac mother plant grown in a greenhouse. In contrast, calli subcultured on Murashige-Skoog media containing combinations of 2,4-dichlorophenoxyacetic acid and kinetin produced only trace amounts of emetic alkaloids.

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Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

IAA:

indole-3-acetic acid

NAA:

l-naphthaleneacetic acid

Kin:

kinetin

MS:

Murashige-Skoog

EM:

emetine

CP:

cephaeline

DW:

dry weight.

References

  • British Pharmacopoeia (1985) XXI 554–557

  • Endo T, Yamada Y (1985) Phytochem 24:1233–1236

    Google Scholar 

  • Janot MM (1953) in: Manske RHF (ed) The Alkaloids, vol 3, Academic Press, New York, pp. 363–394

    Google Scholar 

  • Japanese Pharmacopoeia (1986) XI D679–682

  • Kamada H, Okamura N, Satake M, Harada H, Shimomura K (1986) Plant Cell Reports 5, 239–242

    Google Scholar 

  • Naito N (1984) Farumashia 20:1013–1018

    Google Scholar 

  • Rhodes MJC, Payne J, Robins RJ (1986) Planta Med. 226–229

  • Robins RJ, Payne J, Rhodes MJC (1986) Planta Med. 220–226

  • Seventh-edition Japanese Pharmacopoeia [Comentary book] (1966) Hirokawa Pub. Co. Tokyo, c 997–1001

  • Teshima D, Tsuchiya T, Aoyama T, Horioka M (1984) Iyakuhin Kenkyu 15: 63–71

    Google Scholar 

  • Tsuda H, Umetsu G, Yoshioka T, Iwasaki I (1979) The Japan. J of Acute Medicine 3: 1328–1356

    Google Scholar 

  • United States Pharmacopoeia (1985) XXI 554–557

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Authors and Affiliations

  1. Department of Hospital Pharmacy, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashi-ku, 812, Fukuoka, Japan

    Daisuke Teshima & Toshinobu Aoyama

  2. Tsukuba Medicinal Plant Research Station, National Institute of Hygienic Sciences, 1 Hachimandai, Tsukuba, 305, Ibaraki, Japan

    Kayo Ikeda, Motoyoshi Satake & Koichiro Shimomura

Authors
  1. Daisuke Teshima
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  2. Kayo Ikeda
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  3. Motoyoshi Satake
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  4. Toshinobu Aoyama
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  5. Koichiro Shimomura
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Additional information

Communicated by F. Constabel

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Teshima, D., Ikeda, K., Satake, M. et al. Production of emetic alkaloid by in vitro culture of cephaelis ipecacuanha A. Richard. Plant Cell Reports 7, 278–280 (1988). https://doi.org/10.1007/BF00272542

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  • DOI: https://doi.org/10.1007/BF00272542

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