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Micropropagation of threatened medicinal plant Andrographis lineata var. lawii

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Abstract

Andrographis lineata var. lawii is a threatened and endemic species that yield several therapeutic flavonoids. In the present study, a simple and efficient in vitro plant regeneration protocol was developed using shoot tip, nodal and cotyledonary explants. Shoot tip and nodal explants were involved in direct regeneration of multiple shoots on Murashige and Skoog medium supplemented with benzyl amino purine (BAP). An average of 24.83 and 21.41 shoots were regenerated from shoot tip and nodal explants on MS medium containing 7.5 µM and 10 µM BAP, respectively. Whereas, cotyledonary leaf explants developed callus-mediated multiple shoot buds on thidiazuron (TDZ) supplemented medium from petiolar regions, however, shoot buds did not propagated further. Upon sub-culturing of callus-containing shoot buds on MS medium containing BAP, proliferation and elongation were observed and optimal results were realized on an MS medium supplemented with 2 µM BAP. Histological analysis was carried out to reveal the early events of shoot regeneration. Rooting of shoots was achieved on 1/4 MS medium containing 10 µM indole butyric acid (IBA). The in vitro regeneration methodology is useful for the mass production of plants and germplasm preservation.

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Abbreviations

BAP:

6-Benzyl amino purine

2, 4-D:

2,4-dichlorophenoxyacetic acid

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

NAA:

α-Naphthalene acetic acid

KN:

Kinetin

2-iP:

2-isopentenyl adenine

MS:

Murashige and Skoog medium

TDZ:

Thidiazuron

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Acknowledgements

Hosakatte Niranjana Murthy is thankful for the ?Brain Pool (BP)? program Grant No.2022H1D3A2A02056665.

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  1. Department of Botany, Karnatak University, 580003, Dharwad, India

    Sathish Shekhappa Kadapatti & Hosakatte Niranjana Murthy

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  1. Sathish Shekhappa Kadapatti
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Kadapatti, S., Murthy, H. Micropropagation of threatened medicinal plant Andrographis lineata var. lawii. Vegetos 37, 202–210 (2024). https://doi.org/10.1007/s42535-023-00585-6

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