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Comparative Study on Bone Marrow-Versus Adipose-Derived Stem Cells on Regeneration and Re-Innervation of Skeletal Muscle Injury in Wistar Rats

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Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

Background:

Skeletal muscle injuries are frequent clinical challenges due to associated fibrosis and disability. Regenerative medicine is an emerging promising strategy for such cases. The aim of this study was to compare between the effects of bone marrow-mesenchymal stem cells (BM-MSCs) versus adipose tissue stromal cells (ADSCs) on regeneration and re-innervation of skeletal muscle laceration injury in Wistar rats at different time intervals.

Methods:

Six young male rats were used as a source of allogenic MSCs. Eighty-four adult female rats were divided into: Group I (control), Group II (Untreated Laceration): right gluteal muscle was lacerated and left for spontaneous healing, Group III (BM-MSCs): right gluteal muscle was lacerated with concomitant local intramuscular injection of 1 × 106 BM-MSCs in the lacerated muscle, Group IV (ADSCs): right gluteal muscle was lacerated with concomitant local intramuscular injection of 1 × 106 ADSCs in lacerated muscle. Rats were sacrificed after one, two and eight weeks. Muscles were processed to prepare sections stained with H&E, Mallory’s trichrome and immune-histochemical staining (neurofilament light chain).

Results:

A significant increase in collagen fibers and failure of re-innervation were noticed in untreated laceration group. BM-MSCs-treated groups showed regeneration of muscle fibers but with increased collagen fibers. Meanwhile, ADSCs showed better regenerative effects evidenced by significant increase in the number of myotubes and significant decrease in collagen deposition. Re-innervation was noticed in MSCs-injected muscles after 8 weeks of laceration.

Conclusion:

Both BM-MSCs and ADSCs improved regeneration of skeletal muscle laceration injury at short- and long-term durations. However, fibrosis was less in ADSCs-treated rats. Effective re-innervation of injured muscles occurred only at the long-term duration.

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Authors and Affiliations

  1. Department of Histology and Cell Biology, Faculty of Medicine, Ain Shams University, El-Khalyfa El-Mamoun Street Abbasya, Cairo, Egypt

    Manal H. Moussa, Ghada G. Hamam, Asmaa E. Abd Elaziz, Marwa A. Rahoma, Abeer A. Abd El Samad, Dalia A. A. El-Waseef & Mohamed A. Hegazy

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  1. Manal H. Moussa
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  2. Ghada G. Hamam
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  3. Asmaa E. Abd Elaziz
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  4. Marwa A. Rahoma
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  5. Abeer A. Abd El Samad
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Correspondence to Ghada G. Hamam.

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Ethical statement

The animal experiment was conducted in the Research Center institute (MASRI), Faculty of Medicine, Ain Shams University. The FMASU REC is organized and operated according to guidelines of the International Council on Harmonization (ICH) and the Islamic Organization for Medical Science (IOMS), the United States Office for Human Research Protections and the United States Code of Federal regulations and operates under Federal Wide Assurance N. FWA 00017585.

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Moussa, M.H., Hamam, G.G., Abd Elaziz, A.E. et al. Comparative Study on Bone Marrow-Versus Adipose-Derived Stem Cells on Regeneration and Re-Innervation of Skeletal Muscle Injury in Wistar Rats. Tissue Eng Regen Med 17, 887–900 (2020). https://doi.org/10.1007/s13770-020-00288-y

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  • DOI: https://doi.org/10.1007/s13770-020-00288-y

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