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Local delivery of recombinant human bone morphogenic protein-2 (rhBMP-2) from rhBMP-2/heparin complex fixed to a chitosan scaffold enhances osteoblast behavior

  • Original Article
  • Regenerative Medicine
  • Published:
Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

The objective of this study was to determine the effects on osteoblast activity of a recombinant human bone morphogenic protein (rhBMP)-2/heparin complex immobilized on a chitosan scaffold (Hep-C-rhBMP-2/chitosan) compared to a rhBMP-2-dipping chitosan (rhBMP-2/chitosan) and to a chitosan scaffold alone. Scanning electron microscopy (SEM) confirmed that the surface and cross morphologies of Hep-C-rhBMP-2/chitosan and rhBMP-2/chitosan were similar to those of the chitosan scaffold, and that the Hep-C-rhBMP-2/chitosan scaffold achieved sustained, controlled release of rhBMP-2 compared to the rhBMP-2/chitosan scaffold. We used measurements of alkaline phosphatase activity, calcium deposition, and real-time PCR to determine that significantly more MG-63 cells were successfully cultured on Hep-C-rhBMP-2/chitosan than on rhBMP-2/chitosan or the chitosan scaffold alone. These results suggest that the Hep-C-rhBMP-2/chitosan scaffold promotes osteoblast behavior beyond the other two scaffolds tested. This system may have advantages for dental and orthopedic applications to regenerate bone.

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Authors and Affiliations

  1. Department of Orthopedic Surgery & Rare Diseases Institute, Korea University Medical Center, Guro Hospital, *148, Gurodong-ro, Guro-gu, Seoul, 152-703, Korea

    Young-Pil Yun & Sung Eun Kim

  2. Institute of Cell & Tissue Engineering, Department of Biomedical Sciences, College of Medicine, The Catholic University of Korea, #505 Banpo-dong, Seocho-gu, Seoul, 137-701, Korea

    Dae Hyeok Yang

  3. Department of Oral and Maxillofacial Surgery, School of Dentistry, Kyung Hee University, *1 Hoegi-dong, D ongdaemun-gu, 130-701, Seoul, Korea

    Sun-Wook Kim, Joo-Young Ohe, Baek-Soo Lee & Byung-Joon Choi

  4. Division of Biological Imaging, Chuncheon Center, Korea Basic Science Institute, 192-1 Hyoja 2-dong, Chuncheon, Gangwon-do, 200-701, Korea

    Kyeongsoon Park

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  1. Young-Pil Yun
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  2. Dae Hyeok Yang
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Correspondence to Byung-Joon Choi or Sung Eun Kim.

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These authors contributed equally to this work.

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Yun, YP., Yang, D.H., Kim, SW. et al. Local delivery of recombinant human bone morphogenic protein-2 (rhBMP-2) from rhBMP-2/heparin complex fixed to a chitosan scaffold enhances osteoblast behavior. Tissue Eng Regen Med 11, 163–170 (2014). https://doi.org/10.1007/s13770-014-0049-0

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  • DOI: https://doi.org/10.1007/s13770-014-0049-0

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