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Biodegradation of 3-chloroaniline by suspended cells and biofilm of Acinetobacter baumannii GFJ1

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Abstract

Acinetobacter baumannii strain GFJ1 was isolated from soil using 3-chloroaniline (3CA) as sole of carbon, nitrogen, and energy source under both aerobic and anaerobic conditions. The investigation of aerobic utilization profile showed that the utilization kinetics of 3CA followed the Edward model with a maximum specific degradation as 3.45 ± 0.33 µM.h−1.mg cell protein−1, and apparent half-saturation coefficient value was 0.062 ± 0.01 mM. The aerobic utilization toward 3CA was stimulated with the addition of sodium nitrate and citrate. Under anaerobic conditions, A. baumannii GFJ1 was able to utilize 3CA linked with nitrate reduction. The investigation of biofilm formation showed that biofilm formation was affected by cosubstrates and 3CA concentrations. Biofilm formation enhanced with the presence of cosubstrates, especially nitrogen sources. The biofilm formation and chemical degradation by biofilm increased in the following intervals of incubation with the supply of fresh medium. The results indicate that A. baumannii GFJ1 has a potential for the application to clean up 3CA.

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Acknowledgments

This work was financially supported by the Center of Excellence on Hazardous Substance Management (HSM), Chulalongkorn University, Bangkok, Thailand and the Vietnamese Government.

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Authors and Affiliations

  1. International Program in Hazardous Substance and Environmental Management, Graduate School, Chulalongkorn University, Bangkok, 10330, Thailand

    Ha Danh Duc

  2. Center of Excellence on Hazardous Substance Management (HSM), Chulalongkorn University, Bangkok, 10330, Thailand

    Ha Danh Duc

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  1. Ha Danh Duc
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Correspondence to Ha Danh Duc.

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Duc, H.D. Biodegradation of 3-chloroaniline by suspended cells and biofilm of Acinetobacter baumannii GFJ1. Appl Biol Chem 59, 703–709 (2016). https://doi.org/10.1007/s13765-016-0216-1

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  • DOI: https://doi.org/10.1007/s13765-016-0216-1

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