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Are Aristolochic Acids Responsible for the Chemical Defence of Aposematic Larvae of Battus polydamas (L.) (Lepidoptera: Papilionidae)?

  • Ecology, Behavior and Bionomics
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Abstract

Aristolochic acids (AAs) are thought to be responsible for the chemical protection of the aposematic larvae Battus polydamas (L.) (Papilionidae: Troidini) against predators. These compounds are sequestered by larvae from their Aristolochia (Aristolochiaceae) host plants. Studying the role of the chemical protection of the second and fifth instars of B. polydamas against potential predators, we found that the consumption of larvae by the carpenter ant Camponotus crassus Mayr and young chicks Gallus gallus domesticus was dependent on larval developmental stage. Second instars were more preyed upon than fifth instars; however, the assassin bug Montina confusa Stål was not deterred by chemical defences of the fifth instar B. polydamas. Laboratory bioassays with carpenter ants and young chicks using palatable baits topically treated with a pure commercial mixture of AAs I and AAs II in concentrations up to 100 times those previously found in B. polydamas larvae showed no activity. Similar results were found in field bioassays, where palatable baits treated as above were exposed to the guild of predators that attack B. polydamas larvae and were also consumed irrespective of the commercial AA concentration used. These results suggest that the mixture of AAs I and AAs II have no defensive role against predators, at least against those investigated in the present work. Other compounds present in Aristolochia host plants such as O-glycosylated AAs; benzylisoquinoline alkaloids; and mono-, sesqui-, di-, and triterpenes, which can be sequestered by Troidini, could act as deterrents against predators.

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Acknowledgments

We thank Fundação José Pedro de Oliveira for the permission to work in the Santa Genebra Reserve. Financial support was provided by FAPESP (98/01065-7, 11/17708-0) and CNPq (304969/2009-0) to JRT. This work is part of the Doctor Thesis of ABBM.

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  1. A B B Morais

    Present address: Lab de Ecologia Química, Depto de Biologia Animal, Instituto de Biologia, Univ Estadual de Campinas (UNICAMP), CP 6109, 13083-970, Campinas, SP, Brasil

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  1. Lab de Ecologia Química, Depto de Biologia Animal, Instituto de Biologia, Univ Estadual de Campinas (UNICAMP), CP 6109, 13083-970, Campinas, SP, Brasil

    K S Brown Jr., M A Stanton, K F Massuda & J R Trigo

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Correspondence to J R Trigo.

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Morais, A.B.B., Brown, K.S., Stanton, M.A. et al. Are Aristolochic Acids Responsible for the Chemical Defence of Aposematic Larvae of Battus polydamas (L.) (Lepidoptera: Papilionidae)?. Neotrop Entomol 42, 558–564 (2013). https://doi.org/10.1007/s13744-013-0163-z

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