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Synthesis, characterization and protein separation efficiency of N-isopropylacrylamide-co-N-tertiary butylacrylamide-co-acrylamide-based hydrogels

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Abstract

In the present study, hydrogels were prepared by free radical polymerization in water–dioxane mixture with fixed molar ratio (25 mol%) of N-isopropylacrylamide (NIPAM) and varying remaining molar concentrations of N-tert-butylacrylamide (NTBA) and acrylamide (AAm). The structure of the resultant hydrogels was studied by Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) techniques. The thermal properties of the hydrogels were analyzed by thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC) methods. DSC thermograms were used for the quantitative determination of free, interfacial and bound water contents. The result showed that the free and interfacial water contents increased with increase in the hydrophilic AAm content, and the bound water content increased with hydrophobic NTBA content in the hydrogels. Swelling behavior of the hydrogels was evaluated at different temperatures. The percentage swelling and diffusion kinetic parameters (network structure constant, type of diffusion and diffusion constant) were calculated for all samples. The diffusion was found to be Fickian type for copolymer having equimolar concentrations of NTBA and AAm and non-Fickian type for others. Diffusion coefficients of the hydrogels were found to be increased with increasing temperature. In addition, poly(NIPAM-co-NTBA-co-AAm) hydrogels were used in concentration separation process for BSA solution. The result showed that the copolymer with equimolar NTBA and AAm contents has high separation efficiency with good thermoresponsive behavior among all copolymers.

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Acknowledgments

The authors are grateful to the Central Instrumentation Facility (CIF) at Birla Institute of Technology, Mesra, established in 2006, and the Technical Education Quality Improvement Programme (TEQIP) funded by World Bank for the State of the Art Instrumentation Facility. The authors also acknowledge the financial support from the Central Coir Research Institute (CCRI), Ministry of Micro, Small and Medium Enterprises (MSME) (Government of India), Alappuzha, Kerala, India.

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  1. Department of Chemical and Polymer Engineering, Birla Institute of Technology, Mesra, Ranchi, Jharkhand, 835215, India

    Suman Shekhar, M. Mukherjee & Akhil Kumar Sen

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  1. Suman Shekhar
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  2. M. Mukherjee
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  3. Akhil Kumar Sen
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Correspondence to Akhil Kumar Sen.

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Shekhar, S., Mukherjee, M. & Sen, A.K. Synthesis, characterization and protein separation efficiency of N-isopropylacrylamide-co-N-tertiary butylacrylamide-co-acrylamide-based hydrogels. Iran Polym J 21, 895–905 (2012). https://doi.org/10.1007/s13726-012-0094-2

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  • DOI: https://doi.org/10.1007/s13726-012-0094-2

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