Abstract
Myxofibrosarcoma (MFS) is an aggressive sarcoma with a highly complex karyotype. Complete resection is the only curative treatment for MFS because it is refractory to chemotherapy. To improve clinical outcomes, it is critical to develop novel treatments for MFS. Although patient-derived cell lines play a key role in cancer research, only 12 MFS cell lines have been reported to date, and considering the diversity of the disease, more cell lines need to be established. Hence, in the present study, we established a novel MFS cell line, NCC-MFS4-C1, using a surgically resected tumor tissue from a patient with MFS. NCC-MFS4-C1 cells exhibited copy number alterations similar to those of the original tumors and showed constant proliferation, spheroid formation, and aggressive invasion. By screening a drug library, we found that actinomycin D, bortezomib, docetaxel, eribulin, and romidepsin significantly reduced the proliferation of NCC-MFS4-C1 cells. Therefore, the NCC-MFS4-C1 cell line may be a useful resource for researching MFS.
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Acknowledgements
We thank Drs. F. Nakatani, E. Kobayashi, S. Fukushima, M. Nakagawa, T. Komatsubara, M. Saito, and C. Sato of the Department of Musculoskeletal Oncology, and Drs. M Arakaki, and Y Suemitsu of the Department of Diagnostic Pathology, National Cancer Center Hospital, for sampling tumor tissue specimens from surgically resected materials. We also appreciate the technical assistance provided by Ms. Y. Kuwata (Division of Rare Cancer Research), and the technical support provided by Ms. Y. Shiotani, Mr. N. Uchiya, and Dr. T. Imai (Central Animal Division, National Cancer Center Research Institute). We would like to thank Editage (www.editage.jp) for help with English language editing and constructive comments on the manuscript. This research was technically assisted by the Fundamental Innovative Oncology Core at the National Cancer Center.
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This research was supported by the Japan Agency for Medical Research and Development (grant number 20ck0106537h0001).
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The ethical committee of the National Cancer Center approved the use of clinical materials for this study (approval number 2004–050).
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Yoshimatsu, Y., Noguchi, R., Tsuchiya, R. et al. Establishment and characterization of NCC-MFS4-C1: a novel patient-derived cell line of myxofibrosarcoma. Human Cell 34, 1911–1918 (2021). https://doi.org/10.1007/s13577-021-00589-x
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DOI: https://doi.org/10.1007/s13577-021-00589-x