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Establishment and characterization of NCC-MFS3-C1: a novel patient-derived cell line of myxofibrosarcoma

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Abstract

Myxofibrosarcoma (MFS) is one of the most aggressive sarcomas with highly complex karyotypes and genomic profiles. Although a complete resection is required in the treatment of MFS, it is often not achieved due to its strong invasive nature. Additionally, MFS is refractory to conventional chemotherapy, leading to poor prognosis. Therefore, it is necessary to develop novel treatment modalities for MFS. Patient-derived cell lines are important tools in basic research and preclinical studies. However, only 10 MFS cell lines have been reported to date. Furthermore, among these cell lines, merely two MFS cell lines are publicly available. Hence, we established a novel MFS cell line named NCC-MFS3-C1, using a surgically resected tumor specimen from a patient with MFS. NCC-MFS3-C1 cells had copy number alterations corresponding to the original tumor. NCC-MFS3-C1 cells demonstrate constant proliferation, spheroid formation, and aggressive invasion. In drug screening tests, the proteasome inhibitor bortezomib and the histone deacetylase inhibitor romidepsin demonstrated significant antiproliferative effects on NCC-MFS3-C1 cells. Thus, the NCC-MFS3-C1 cell line is a useful tool in both basic and preclinical studies for MFS.

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Acknowledgements

We thank Drs. F. Nakatani, E. Kobayashi, S. Fukushima, M. Nakagawa, T. Komatsubara, M Saito, C. Sato (Department of Musculoskeletal Oncology), Drs. T Shibayama, and H. Tanaka (Department of Diagnostic Pathology), National Cancer Center Hospital, for sampling tumor tissue specimens from surgically resected materials. We also appreciate the technical assistance provided by Ms. Y. Kuwata (Division of Rare Cancer Research). We appreciate the technical support provided by Ms. Y. Shiotani, Mr. N. Uchiya, and Dr. T. Imai (Central Animal Division, National Cancer Center Research Institute). We would like to thank Editage (www.editage.jp) for help with English language editing and constructive comments on the manuscript. This research was technically assisted by the Fundamental Innovative Oncology Core at the National Cancer Center.

Funding

This research was supported by the Japan Agency for Medical Research and Development (Grant number 20ck0106537h0001).

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Authors and Affiliations

  1. Division of Rare Cancer Research, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo, 104-0045, Japan

    Ryuto Tsuchiya, Yuki Yoshimatsu, Rei Noguchi, Yooksil Sin, Takuya Ono, Akane Sei & Tadashi Kondo

  2. Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba, 260-8670, Japan

    Ryuto Tsuchiya & Seiji Ohtori

  3. Department of Translational Oncology, Fundamental Innovative Oncology Core Center, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo, 104-0045, Japan

    Fumitaka Takeshita

  4. Department of Musculoskeletal Oncology, National Cancer Center Hospital, 5-1-1 Tsukiji, Chuo-ku, Tokyo, 104-0045, Japan

    Jun Sugaya, Shintaro Iwata & Akira Kawai

  5. Department of Diagnostic Pathology, National Cancer Center Hospital, 5-1-1 Tsukiji, Chuo-ku, Tokyo, 104-0045, Japan

    Akihiko Yoshida

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  1. Ryuto Tsuchiya
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Correspondence to Tadashi Kondo.

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The authors declare that they have no conflict of interest.

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The ethical committee of the National Cancer Center approved the use of clinical materials for this study (approval number 2004–050).

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Written informed consent for publication was provided by the patient.

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Supplementary Information

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Supplementary file1 Short tandem repeat analysis (TIF 214 kb)

13577_2021_548_MOESM2_ESM.tif

Supplementary file2 Tumorigenesis in nude mice. (A) NCC-MFS3-C1 cells transplanted into BALB/c nude mice formed a small tumor mass under the described condition. One of the four masses showed areas of (B) dense proliferation of atypical oval cells and (C) myxoid components in Hematoxylin and eosin staining sections. (D) The graph showing estimated tumor volume. Bars represent the mean standard error (TIF 1984 kb)

Supplementary file3 Growth curves for the IC50 value calculations of 24 anticancer agents (TIF 600 kb)

Supplementary file4 (XLSX 24 kb)

Supplementary file5 (XLSX 166 kb)

Supplementary file6 (XLSX 25 kb)

Supplementary file7 (XLSX 11 kb)

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Tsuchiya, R., Yoshimatsu, Y., Noguchi, R. et al. Establishment and characterization of NCC-MFS3-C1: a novel patient-derived cell line of myxofibrosarcoma. Human Cell 34, 1266–1273 (2021). https://doi.org/10.1007/s13577-021-00548-6

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  • DOI: https://doi.org/10.1007/s13577-021-00548-6

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