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Experimental design, modeling, and optimization of production of xylooligosaccharides by hydrothermal pretreatment of sugarcane bagasse and straw

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Abstract

Hydrothermal pretreatment (HP) of two by-products of sugarcane, bagasse (SB) and straw (SS), was optimized to produce xylooligosaccharides (XOS). A central composite rotational design (CCRD) in conjunction with response surface methodology was used to optimize the conditions for maximum XOS production. The developed mathematical models were statistically adequate to predict xylan conversion to XOS, and the by-products were promising for the production of XOS. For both by-products, the mass load in the optimal experiments was of 10%. The xylan conversion to XOS was 24.8% for SB and 45.3% for SS, XOS yield of 53.3 and 96 mg.g−1, respectively. The SB produced more XOS with a greater degree of polymerization than SS. Sugarcane by-products are important in the production of XOS, which may be a valuable commercial product. Furthermore, cellulose and lignin-enriched solids recovered following the optimized HP conditions can be fractionated to obtain other bioproducts of commercial interest.

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Abbreviations

HPLC:

High-performance liquid chromatography system

C18 :

Reverse phase column HYPERSIL C18

SB:

Sugarcane bagasse

SS:

Sugarcane straw

XOS:

Xylooligosaccharides

HP:

Hydrothermal pretreatment

X1 :

Xylose

X2 :

Xylobiose

X3 :

Xylotriose

X4 :

Xylotetraose

X5 :

Xylopentaose

X6 :

Xylohexaose

X7 :

Xyloheptaose

X8 :

Xylooctaose

X9 :

Xylonanose

X6 :

Oligosaccharides with more than six repetitive units

log R 0 :

Severity factor

CCRD:

Central composite rotational design

HH:

Hemicellulosic hydrolysate

NaOH:

Sodium hydroxide

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Funding

São Paulo State Research Support Foundation (FAPESP, contract number 2018/06241–3) funded this work. Coordination of Improvement of Higher Education Personnel (CAPES) funded the doctoral scholarship of Lídia Manfrin Dias.

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Authors and Affiliations

  1. School of Pharmaceutical Sciences (FCF), Department of Bioprocess Engineering and Biotechnology, São Paulo State University (UNESP), Araraquara-SP, Brazil

    Lídia Manfrin Dias, Flávia Sanchez Penalva P. Neto, Samuel Conceição de Oliveira & Fernando Masarin

  2. Bioenergy Research Institute (IPBEN), São Paulo State University (UNESP), Rio Claro-SP, Brazil

    Michel Brienzo

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  1. Lídia Manfrin Dias
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Contributions

LMD and FN performed HP in SB and SS and chemically characterized the recovered liquid and solid fractions. LMD, FN, MB, SCO, and FM participated in the design of the experiments, data interpretation, and mathematical modeling. LMD, FN, SCO, MB, and FM participated in reviewing the manuscript and data interpretation. All authors read and approved the final manuscript.

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Correspondence to Fernando Masarin.

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Dias, L.M., Neto, F.S.P.P., Brienzo, M. et al. Experimental design, modeling, and optimization of production of xylooligosaccharides by hydrothermal pretreatment of sugarcane bagasse and straw. Biomass Conv. Bioref. 13, 12777–12794 (2023). https://doi.org/10.1007/s13399-021-02151-z

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