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The Use of Palm Oil-Based Waste Cooking Oil to Enhance the Production of Polyhydroxybutyrate [P(3HB)] by Cupriavidus necator H16 Strain

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A Correction to this article was published on 21 March 2018

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Abstract

Waste cooking oil (WCO) is of increasing interest as an inexpensive feedstock to produce biodegradable plastic, poly(3-hydroxybutyrate) [P(3HB)]. In the present study, palm oil-based WCO (PO-WCO) was obtained from nine different locations. Palm oil-based fresh cooking oil (PO-FCO) and PO-WCO were characterised via proximate and physicochemical analysis, prior to being used as carbon sources for the biosynthesis of P(3HB) using Cupriavidus necator H16. It was shown that the free fatty acid, peroxide value, and saturated compounds in all batches of PO-WCO were higher compared to those in the PO-FCO. The cells produced 60–80 wt% P(3HB) with dry cell weight of 14–17 g/L. The weight average molecular weight \((M_{\mathrm{w}})\) was found to be \(1.8 \times 10^{6}\) Da with a polydispersity \((M_{\mathrm{w}}/M_{\mathrm{n}})\) of 2.7 when PO-WCO was used as the carbon source. The PO-WCO was found to be suitable to be used as a sustainable carbon source for cell growth and P(3HB) biosynthesis.

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Change history

  • 21 March 2018

    The original version of this article unfortunately contained a mistake. The presentation of Fig. 3 was incorrect. The corrected figure is given below.

  • 21 March 2018

    The original version of this article unfortunately contained a mistake. The presentation of Fig.?3 was incorrect. The corrected figure is given below.

  • 21 March 2018

    The original version of this article unfortunately contained a mistake. The presentation of Fig.?3 was incorrect. The corrected figure is given below.

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Acknowledgements

The authors thank the community of Teluk Bahang, Penang, Malaysia, for contributing the PO-WCO used in this study. This study was funded in parts by the Division of Industry and Community Network and Short-Term Research Grants (304/Pbiologi/6311070) from Universiti Sains Malaysia (USM). H. Kamilah acknowledges USM Fellowship during the period of the study.

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Authors and Affiliations

  1. Food Technology Division, School of Industrial Technology, Universiti Sains Malaysia, 11800, Gelugor, Penang, Malaysia

    Hanisah Kamilah & Tajul Aris Yang

  2. Department of Water and Environmental Engineering, Faculty of Civil and Environmental Engineering, Universiti Tun Hussein Onn Malaysia (UTHM), 86400 Parit Raja, Batu Pahat, Johor, Malaysia

    Adel Al-Gheethi

  3. Ecobiomaterial Research Laboratory, School of Biological Sciences, Universiti Sains Malaysia, 11800, Gelugor, Penang, Malaysia

    Kumar Sudesh

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  1. Hanisah Kamilah
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  2. Adel Al-Gheethi
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  4. Kumar Sudesh
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Correspondence to Hanisah Kamilah.

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Kamilah, H., Al-Gheethi, A., Yang, T.A. et al. The Use of Palm Oil-Based Waste Cooking Oil to Enhance the Production of Polyhydroxybutyrate [P(3HB)] by Cupriavidus necator H16 Strain. Arab J Sci Eng 43, 3453–3463 (2018). https://doi.org/10.1007/s13369-018-3118-1

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