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Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Cupriavidus necator from waste rapeseed oil using propanol as a precursor of 3-hydroxyvalerate

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Abstract

Waste rapeseed oil is a useful substrate for polyhydroxyalkanoates (PHA) production employing Cupriavidus necator H16. In fed-batch mode, we obtained biomass and PHA yields of 138 and 105 g l−1, respectively. Yield coefficient and volumetric productivity were 0.83 g PHA per g oil and 1.46 g l−1 h−1, respectively. Propanol at 1% (v/v) enhanced both PHA and biomass formation significantly and, furthermore, resulted in incorporation of 3-hydroxyvalerate units into PHA structure. Thus, propanol can be used as an effective precursor of 3-hydroxyvalarete for production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer. During the fed-batch cultivation, propanol concentration was maintained at 1% which resulted in 8% content of 3-hydroxyvalerate in copolymer.

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Acknowledgments

This work was supported by projects MSM 0021630501 and CZ.1.05/2.1.00/01.0012 of the Czech Ministry of Education.

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Authors and Affiliations

  1. Faculty of Chemistry, Department of Food Chemistry and Biotechnology, Brno University of Technology, Purkynova 118, 612 00, Brno, Czech Republic

    Stanislav Obruca, Ivana Marova & Ondrej Snajdar

  2. Faculty of Chemistry, Department of Chemistry and Technology of Environmental Protection, Brno University of Technology, Purkynova 118, 612 00, Brno, Czech Republic

    Ludmila Mravcova

  3. Research Institute of Brewing and Malting, Malting Institute Brno, Mostecka 7, 614 00, Brno, Czech Republic

    Zdenek Svoboda

Authors
  1. Stanislav Obruca
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  2. Ivana Marova
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  3. Ondrej Snajdar
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  4. Ludmila Mravcova
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  5. Zdenek Svoboda
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Correspondence to Stanislav Obruca.

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Obruca, S., Marova, I., Snajdar, O. et al. Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Cupriavidus necator from waste rapeseed oil using propanol as a precursor of 3-hydroxyvalerate. Biotechnol Lett 32, 1925–1932 (2010). https://doi.org/10.1007/s10529-010-0376-8

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  • DOI: https://doi.org/10.1007/s10529-010-0376-8

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