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Improved Lactic Acid Production by In Situ Removal of Lactic Acid During Fermentation and a Proposed Scheme for Its Recovery

  • Research Article - Biological Sciences
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Abstract

Extractive fermentation with 155.8 g/L initial glucose and 364 g of anion exchange resin yielded a 1.2-fold increase in total lactate produced and a 5.9-fold increase in productivity compared with standard batch fermentation at the same glucose concentration. The addition of resin also served as a pH control strategy. Elution of the resin-bound lactate using 1 M HCl at 0.1 bed volume/min resulted in complete recovery of lactate. Further extraction of the eluted lactate and lactate in fermentation broth was conducted with reactive extraction using trioctylamine in octanol. The extraction also provided partial purification of the product, as protein from fermentation broth did not migrate to the extracted solution with the lactate. The recovery of lactate was up to 70 % of the total lactate produced.

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Authors and Affiliations

  1. Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen, 40002, Thailand

    Mallika Boonmee & Onanong Cotano

  2. Fermentation Research Center for Value Added Agricultural Products, Khon Kaen University, Khon Kaen, 40002, Thailand

    Mallika Boonmee

  3. Department of Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand

    Sittipong Amnuaypanich

  4. Department of Chemical Engineering, Faculty of Engineering, Thammasat University, Bangkok, 12121, Thailand

    Nurak Grisadanurak

Authors
  1. Mallika Boonmee
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  2. Onanong Cotano
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  3. Sittipong Amnuaypanich
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  4. Nurak Grisadanurak
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Correspondence to Mallika Boonmee.

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Boonmee, M., Cotano, O., Amnuaypanich, S. et al. Improved Lactic Acid Production by In Situ Removal of Lactic Acid During Fermentation and a Proposed Scheme for Its Recovery. Arab J Sci Eng 41, 2067–2075 (2016). https://doi.org/10.1007/s13369-015-1824-5

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  • DOI: https://doi.org/10.1007/s13369-015-1824-5

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