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Recovery of lactic acid from simultaneous saccharification and fermentation media using anion exchange resins

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Abstract

The physicochemical properties (capacity, kinetics and selectivity) of the ion exchange resins Amberlite IRA900, IRA400, IRA96 and IRA67 were determined to evaluate their comparative suitability for lactic acid recovery. Both the kinetics of lactic acid sorption from aqueous solutions and the equilibrium were assessed using mathematical models, which provided a close interpretation of the experimental results. The best resins (Amberlite IRA96 and IRA67) were employed in further fixed-bed operation using aqueous lactic acid solutions as feed. In this set of experiments, parameters such as capacity, regenerant consumption, percentage of lactic acid recovery and product concentration were measured. Amberlite IRA67, a weak base resin, was selected for lactic acid recovery from SSF (simultaneous saccharification and fermentation) broths. Owing to the presence of nutrients and ions other than lactate, a slightly decreased capacity was determined when using SSF media instead aqueous lactic acid solutions, but quantitative lactic acid recoveries at constant capacities were obtained in four sequential load/regeneration cycles.

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References

  1. Evangelista RL, Nikolov ZL (1996) Recovery and purification of lactic acid from fermentation broth by adsorption. Appl Biochem Biotechnol 57–58:471–480

  2. Kaufman EN, Cooper SP, Davison BH (1994) Screening of resins for use in a biparticle fluidized-bed bioreactor for the continuous fermentation and separation of lactic acid. Appl Biochem Biotechnol 45–46:545–554

  3. Zheng Y, Ding X, Cen P, Yang CW, Tsao GT (1996) Lactic acid fermentation and adsorption on PVP. Appl Biochem Biotechnol 57–58:627–632

  4. Hughes CH (1994) Guía de aditivos. Acribia, Zaragoza, Spain

  5. Kaufman EN, Cooper SP, Clement SL, Little MH (1995) Use of a biparticle fluidized-bed bioreactor for continuous and simultaneous fermentation and purification of lactic acid. Appl Biochem Biotechnol 45–46:605–620

  6. Zihao W, Kefeng Z (1995) Kinetics and mass transfer for lactic acid recovery with anion exchange method in fermentation solution. Biotechnol Bioeng 47:1–7

    Google Scholar 

  7. Evangelista RL, Mangold AJ, Nikolov ZL (1994) Recovery of lactic acid by sorption: resin evaluation. Appl Biochem Biotechnol 45–46:131–144

  8. Nomura Y, Iwahara M, Hongo M (1987) Lactic acid production by electrodialysis fermentation using immobilized growing cells. Biotechnol Bioeng 30:788–793

    CAS  Google Scholar 

  9. Davison BH, Scott CD (1992) A proposed biparticle fluidised-bed for lactic acid fermentation and simultaneous adsorption. Biotecnol Bioeng 39:365–368

    CAS  Google Scholar 

  10. Moldes AB, Alonso JL, Parajó JC (2000) Multi-step feeding systems for lactic acid production by simultaneous saccharification and fermentation of processed wood. Bioproc Eng 22:175–180

    Article  CAS  Google Scholar 

  11. Mafart P, Béliard E (1994) Ingeniería industrial alimentaria—II. Técnicas de separación. Acribia, Zaragoza

  12. Harland CE (1994) Ion exchange: theory and practice. Royal Society of Chemistry, Cambridge

    Google Scholar 

  13. Barboza M, Almeida RMRG, Hokka CO (2002) Kinetic studies of clavulanic acid recovery by ion exchange chromatography. Bioseparation 10:221–227

    Article  Google Scholar 

Download references

Acknowledgements

The authors are grateful to "Xunta de Galicia" for the financial support of this work in the scope of the Research Project reference PGIDT00PXI38301PR.

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Authors and Affiliations

  1. Department of Chemical Engineering, Faculty of Science, University of Vigo (Campus Ourense), As Lagoas, 32004 , Ourense, Spain

    A. B. Moldes, J. L. Alonso & J. C. Parajó

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  1. A. B. Moldes
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  2. J. L. Alonso
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  3. J. C. Parajó
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Correspondence to J. L. Alonso.

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Moldes, A.B., Alonso, J.L. & Parajó, J.C. Recovery of lactic acid from simultaneous saccharification and fermentation media using anion exchange resins. Bioprocess Biosyst Eng 25, 357–363 (2003). https://doi.org/10.1007/s00449-002-0316-7

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  • DOI: https://doi.org/10.1007/s00449-002-0316-7

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