Skip to main content
SpringerLink
Log in

In situ separation of lactic acid from fermentation broth using ion exchange resins

  • Original Paper
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Lactic acid fermentation is an end product inhibited reaction. In situ separation of lactic acid from fermentation broth using ion exchange resins was investigated and compared with conventional fermentation system. Amberlite resin (IRA-400, Cl−) was used to separate lactic acid from fermentation broth and pH was controlled online with an automatic pH controller. The effect of process variables on lactic acid production by Lactobacillus casei in whey permeate was studied. The maximum productivity was obtained at pH = 6.1, T = 37 °C and impeller speed = 200 rpm. The maximum concentration of lactic acid at optimum condition was found to be 37.4 g/L after 38 h of fermentation using in situ separation system. The productivity of in situ separation system was five times increased in comparison with conventional system.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Ataei SA, Sh Dolatshahi (2005) Isolation and determination of the most appropriate microbial strain and fermentation condition for single cell protein production from whey. J Res Health Sci 5:54–58

    Google Scholar 

  2. Barrera DA (1993) Synthesis and characterization of a novel biodegradable polymer Poly (lactic acid-Co-lysine). DAI-B 54(6):3096–3099

    Google Scholar 

  3. Dunn RL, English JP, Strobel JD, Cowsar DR, Tice TR (1988) Preparation and evaluation of lactide/glycolide co polymers for drug delivery. In: Migliaresi C (ed) Polymers in medicine III. Elsevier, Amsterdam, pp 149–159

    Google Scholar 

  4. Faith WL (1993) Encyclopedia of chemical technology. Interscience Pub, Dublin, pp 576–577

    Google Scholar 

  5. Gonzalez MI, Alvarez S, Riera F, Alvarez R (2007) Economic evaluation of an integrated process for lactic acid production from ultrafiltered whey. J Food Eng 80:553–561

    Article  CAS  Google Scholar 

  6. Hatzini KDG, Wang HY (1992) Extractive fermentation systems for organic acids production. Can J Chem Eng 70:543–552

    Article  Google Scholar 

  7. Holland SJ, Tighe BJ, Gould PL (1986) Polymers for biodegradable medical devices I. The potential of polymers as controlled macromolecular release system. J Control release 4:155–160

    Article  CAS  Google Scholar 

  8. Huang H, Yang ST, Ramey DE (2004) A hollow-fiber membrane extraction process for recovery and separation of lactic acid from aqueous solution. Appl Biochem Biotechnol 114:671–688

    Article  Google Scholar 

  9. Jarvinen M, Mylikoski L, Keiski R, Sohlo J (2000) Separation of lactic acid from fermentation broth by reactive extraction. Bioseparation 9:163–166

    Article  PubMed  CAS  Google Scholar 

  10. Krischke W, Schroder M, Trosch W (1991) Continuous production of L-lactic acid from whey permiate by immobilized Lactobacillus casei subsp. casei. Appl Microbiol Biotechnol 34:573–578

    Article  CAS  Google Scholar 

  11. Lewis D (1990) Biodegradable polymers and drug delivery systems. Derkker, Newyork, pp 203–208

    Google Scholar 

  12. Lipinsky ES, sinclair RG (1986) Is lactic acid a commodity chemical? Chem Eng Prog 82:26–32

    CAS  Google Scholar 

  13. Matsumoto M, Kondo K (1998) Fermentation of lactic acid combined with solvent extraction or membrane separation containing a mobile carrier. Recent Res Dev Ferment Bioeng 1:241–252

    CAS  Google Scholar 

  14. Nolan-Itu Pty Ltd (2002) Prepared in association with Excel Pals Australia. Biodegradable plastic Developments and Environmental

  15. Pirt SJ (1985) Effect of chemical inhibition and activation of growth. Principles of microbe and cell cultivation. Blackwell Scientific Publications, Oxford, pp 170–185

    Google Scholar 

  16. Roucourt A, Girard D, Prigent Y (1989) Continuous lactic acid fermentation with cell recycled by ultrafiltration and lactate separation by electrodialysis: model identification. Appl Environ Microbiol 30:528–534

    Google Scholar 

  17. Roukas T, Kotzekidou P (1991) Production of lactic acid from deprotonized whey by Coimoblized Lactobacillus casei and Lactococcus lactic cells. Enzyme Microb Technol 13:33–38

    Article  CAS  Google Scholar 

  18. Thang VH, Koschuh W (2004) Desalination of high salt content mixture by two-stage electrodialysis as the first step of separation valuable substances from grass silage. Desalination 162:343–353

    Article  CAS  Google Scholar 

  19. The American type culture collection (ATCC) (1993)

  20. Thimmaiah SR (2004) Standard methods of biochemical analysis. Kalyani publishers, New Delhi, India, pp 49–57

    Google Scholar 

  21. Tong Y, Hirata M, Takanashi H, Hano T, Kubota F, Goto M, Nakashio F, Matsumoto M (1998) Extraction of lactic acid from fermented broth with microporous hollow fiber membranes. J Memb Sci 143:81–91

    Article  CAS  Google Scholar 

  22. Vaccari G, Gonzalez-vara A, Gampi AL, Dosi E, Brigid P, Matteuzzi D (1993) Fermentative Production of l Lactic acid by Lactobacillus casei DSM 20011 and product recovery using ion exchange resin. Appl Microbiol Biotecnol 40:23–27

    CAS  Google Scholar 

  23. Wang HY (1983) Integrating biochemical separation and purification steps in fermentation Process. Ann NY Acad Sci 413:313–321

    Article  PubMed  CAS  Google Scholar 

  24. Wardel JM, King CJ (1978) Solvent equilibria for extraction of carboxilic acid from water. J Chem Eng Data 23:144–150

    Article  Google Scholar 

Download references

Acknowledgment

We thank Razi institute for providing Lactobacillus casei and Bacillus subtillis strains.

Author information

Authors and Affiliations

  1. Chemical Engineering Department, Tarbiat Modares University, P.O. Box 14115-143, Tehran, Iran

    Seyed Ahmad Ataei & Ebrahim Vasheghani-Farahani

  2. Chemical Engineering Department, Shahid Bahonar University of Kerman, Kerman, Iran

    Seyed Ahmad Ataei

Authors
  1. Seyed Ahmad Ataei
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ebrahim Vasheghani-Farahani
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ebrahim Vasheghani-Farahani.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ataei, S.A., Vasheghani-Farahani, E. In situ separation of lactic acid from fermentation broth using ion exchange resins. J Ind Microbiol Biotechnol 35, 1229–1233 (2008). https://doi.org/10.1007/s10295-008-0418-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10295-008-0418-6

Keywords

Search

Navigation