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Low-frequency fluctuation characteristics in rhesus macaques with SIV infection: a resting-state fMRI study

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Abstract

Simian immunodeficiency virus (SIV)-infected macaque is a widely used model to study human immunodeficiency virus. The purpose of the study is to discover the amplitude of low-frequency fluctuation (ALFF) and fractional ALFF (fALFF) changes in SIV-infected macaques. Seven rhesus macaques were involved in the longitudinal MRI scans: (1) baseline (healthy state); (2) SIV infection stage (12 weeks after SIV inoculation). ALFF and fALFF were subsequently computed and compared to ascertain the changes caused by SIV infection. Whole-brain correlation analysis was further used to explore the possible associations between ALFF/fALFF values and immune status parameters (CD4+ T cell counts, CD4/CD8 ratio and virus load). Compared with the baseline, macaques in SIV infection stage displayed strengthened ALFF values in left precuneus, postcentral gyrus, and temporal gyrus, and weakened ALFF values in orbital gyrus and inferior temporal gyrus. Meanwhile, increased fALFF values were found in left superior frontal gyrus, right precentral gyrus, and superior temporal gyrus, while decreased fALFF values existed in left hippocampus, left caudate, and right inferior frontal gyrus. Furthermore, ALFF and fALFF values in several brain regions showed significant relationships with CD4+ T cell counts, CD4/CD8 ratio, and plasma virus load. Our findings could promote the understanding of neuroAIDS caused by HIV infection, which may provide supplementary evidences for the future therapy study in SIV model.

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Acknowledgments

The authors would thank all patients and participants in the research and Prof. Yufeng Zang (Hangzhou Normal University) for many important scientific discussions about the resting-state fMRI.

Funding

The work was financially supported by the Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding Support [grant number ZYLX201511] and the National Nature Science of Foundation of China [grant number 81771806]. Bin Jing was financially supported by the Beijing Natural Science Foundation [number 7174282].

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Author notes

  1. Jing Zhao and Feng Chen contributed equally to the paper.

Authors and Affiliations

  1. Department of Radiology, Beijing Youan Hospital, Capital Medical University, No.8, Xi Tou Tiao, You An MenWai, Feng Tai District, Beijing, 100069, China

    Jing Zhao, Feng Chen, Meiji Ren, Li Li & Hongjun Li

  2. Department of Infectious Diseases, Beijing Youan Hospital, Capital Medical University, No.8, Xi Tou Tiao, You An Men Wai, Feng Tai District, Beijing, 10069, China

    Aixin Li

  3. School of Biomedical Engineering, Capital Medical University, No.10, Xi Tou Tiao, You An Men Wai, Feng Tai District, Beijing, 100069, China

    Bin Jing

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  1. Jing Zhao
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  2. Feng Chen
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  3. Meiji Ren
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  4. Li Li
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  5. Aixin Li
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  6. Bin Jing
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  7. Hongjun Li
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Correspondence to Bin Jing or Hongjun Li.

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The experiment was conducted according to the guide of Laboratory Animals and approved by the Institutional Animal Care and Use Committee of the Institute of Laboratory Animal Science, CAMS & PUMC.

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The authors declare that they have no conflict of interest.

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Zhao, J., Chen, F., Ren, M. et al. Low-frequency fluctuation characteristics in rhesus macaques with SIV infection: a resting-state fMRI study. J. Neurovirol. 25, 141–149 (2019). https://doi.org/10.1007/s13365-018-0694-5

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