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Characterization of a 5HS-7DS.7DL wheat-barley translocation line and physical mapping of the 7D chromosome using SSR markers

  • Plant Genetics • Original Paper
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Abstract

A spontaneous wheat-barley translocation line was previously detected in the progenies of the Mv9kr1 × ‘Igri’ wheat-barley hybrid and the translocation was identified as 5HS-7DS.7DL. Multicolor genomic in situ hybridization (mcGISH) with D and H genomic DNA probes and three-color fluorescence in situ hybridization (FISH) with repetitive DNA probes (Afa-family, pSc119.2, and pTa71) were performed to characterize the rearranged chromosome. The effect of 5HS and the deleted 7DS fragment on the morphological traits (plant height, fertility, yield, and spike characteristics) of wheat was assessed. Despite the non-compensating nature of the translocation, the plants showed good viability. The aim of the study was to physically localize SSR markers to the telomeric and subtelomeric regions of the 7DS chromosome arm. Of the 45 microsatellite markers analyzed, ten (Xbarc0184, Xwmc0506, Xgdm0130, Xgwm0735, Xgwm1258, Xgwm1123, Xgwm1250, Xgwm1055, Xgwm1220, and Xgwm0635) failed to amplify any 7DS-specific fragments, signaling the elimination of a short chromosome segment in the telomeric region. The breakpoint of the 5HS-7DS.7DL translocation appeared to be more distal than that of reported deletion lines, which provides a new physical landmark for future deletion mapping studies.

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Acknowledgments

This study was funded from the Agrisafe program (EU-FP7-REGPOT 2007–1), the Hungarian National Research Fund (K104382), and from the TÁMOP project (4.2.2.A-11/1/KONV-2012-0064). The authors gratefully acknowledge the excellent technical assistance of A. Heber (IPK-Gatersleben, Germany), and J. Bucsi and F. Tóth (MTA ATK, Hungary). Thanks are due to B. Hooper for revising the manuscript linguistically.

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Authors and Affiliations

  1. Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Brunszvik 2, 2462, Martonvásár, Hungary

    Klaudia Kruppa, Adél Sepsi, Éva Szakács & Márta Molnár-Láng

  2. Leibniz Institute of Plant Genetics and Crop Plant Research (IPK-Gatersleben), Corrensstrasse 3, 06466, Gatersleben, Germany

    Marion S. Röder

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  1. Klaudia Kruppa
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  2. Adél Sepsi
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Correspondence to Márta Molnár-Láng.

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Kruppa, K., Sepsi, A., Szakács, É. et al. Characterization of a 5HS-7DS.7DL wheat-barley translocation line and physical mapping of the 7D chromosome using SSR markers. J Appl Genetics 54, 251–258 (2013). https://doi.org/10.1007/s13353-013-0152-2

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