Abstract
We present a high density physical map of homoeologous group 7 chromosomes from Triticum aestivum L. using a series of 54 deletion lines, 6 random amplified polymorphic DNA (RAPD) markers and 91 cDNA or genomic DNA clones from wheat, barley and oat. So far, 51 chromosome segments have been distinguished by molecular markers, and 54 homoeoloci have been allocated among chromosomes 7A, 7B and 7D. The linear order of molecular markers along the chromosomes is almost identical in the A- B- and D-genome of wheat. In addition, there is colinearity between the physical and genetic maps of chromosomes 7A, 7B and 7D from T. aestivum, indicating gene synteny among the Triticeae. However, comparison of the physical map of chromosome 7D from T. aestivum with the genetic map from Triticum tauschii some markers have been shown to be physically allocated with distortion in more distal chromosome regions. The integration of genetic and physical maps could assist in estimating the frequency and distribution of recombination in defined regions along the chromosome. Physical distance did not correlate with genetic distance. A dense map facilitates the detection of multiple rearrangements. We present the first evidence for an interstitial inversion either on chromosome arm 7AS or 7DS of Chinese Spring. Molecularly tagged chromosome regions (MTCRs) provide landmarks for long-range mapping of DNA fragments.
Similar content being viewed by others
References
Anderson JA, Ogihara Y, Sorrells ME, Tanksley SD (1992) Development of a chromosomal arm map for wheat based on RFLP markers. Theor Appl Genet 83:1035–1043
Appels R, Moran LB (1984) Molecular analysis of alien chromatin introduced into wheat. Stadler Genet Symp 16:529–557
Berhan AM, Hulbert SC, Butler LG, Bennetzen JL (1993) Structure and evolution of the genomes of Sorghum bicolor and Zea mays. Theor Appl Genet 86:598–604
Chao S, Sharp PJ, Worland AJ, Warham EJ, Koebner RMD, Gale MD (1989) RFLP-based genetic maps of wheat homoeologous group 7 chromosomes. Theor Appl Genet 78:495–504
Cheung WY, Chao S, Gale MD (1991) Long-range physical mapping of the α-amylase 1 (α — Amy-1) loci on homoeologous group 6 chromosomes of wheat and rye. Mol Gen Genet 229:373–379
Curtis CA, Lukaszewski AJ (1991) Genetic linkage between C-bands and storage protein genes in chromosome 1B of tetraploid wheat. Theor Appl Genet 81:245–252
Devos KM, Atkinson MD, Chinoy CN, Francis HA, Harcourt RL, Koebner RMD, Liu CJ, Masojc P, Xie DX, Gale MD (1993) Chromosomal rearrangements in the rye genome relative to that of wheat. Theor Appl Genet 85:673–680
Dvorak J, Chen KC (1984) Distribution of nonstructural variation between wheat cultivars along chromosome 6Bp: evidence from the linkage map and the physical map of the arm. Genetics 106:325–333
Endo TR (1986) Complete identification of common wheat chromosomes by means of C-banding technique. Jpn J Genet 61:89–93
Endo TR (1988) Induction of chromosomal structural changes by a chromosome of Aegilops cylindrica L. in common wheat. J Hered 79:366–370
Endo TR, Gill BS (1993) Production of deletion stocks in common wheat. Proc 8th Int Wheat Genet Symp Bejing, China (in press)
Friebe B, Mukai Y, Gill BS (1992) C-banding polymorphisms in several accessions of Triticum tauschii (Aegilops squarrosa). Genome 35:192–199
Gale MD (1993) Chromosome group reports: Group 7. In: Hoisington D, McNab A (eds) Progress in genome mapping of wheat and related species. Proc 3rd Publ Workshop International Triticeae Mapping Initiative. CIMMYT Mexico City, pp 103–107
Gale MD, Chao S, Sharp P (1990) RFLP maping in wheatprogress and problems. In: Gustafson JP (ed) Gene manipulation in plant improvement II. Plenum Press, New York, pp 353–363
Gill BS, Chen PD (1987) Role of cytoplasm-specific introgression in the evolution of polyploid wheats. Proc Natl Acad Sci USA 84:6800–6804
Gill KS, Lubbers EL, Gill BS, Raupp WJ, Cox TS (1991) A genetic linkage map of Triticum tauschii (DD) and its relationship to the D genome of bread wheat (AABBDD). Genome 34:362–374
ill KS, Gill BS, Endo TR (1993) A chromosome region-specific mapping strategy reveals gene-rich telomeric ends in wheat. Chromosoma 102:374–381
Helentjaris T, Webber D, Wright S (1988) Identification of genomic locations of duplicated sequences in maize by analysis of restriction fragment length polymorphism. Genetics 118:353–363
Hohmann U, Lagudah ES (1993) C-banding polymorphism and linkage of nonhomologous RFLP loci in the D-genome progenitor of wheat. Genome 36:235–243
Hulbert SH, Richter TE, Axtell JD, Bennetzen JL (1990) Genetic mapping and characterization of Sorghum and related crops by means of maize DNA probes. Proc Natl Acad Sci USA 87:4251–4255
Jampates R, Dvorak J (1986) Location of the Ph locus in the metaphase chromosome map and the linkage map of the 5Bq arm of wheat. Can J Genet Cytol 28:511–519
Keim P, Diers BW, Olson TC, Shoemaker RC (1990) RFLP mapping in soybeans: association between marker loci and variation in quantitative traits. Genetics 126:735–742
Kim NS, Armstrong K, Knott DR (1993) Molecular detection of Lophopyrum chromatin in wheat-Lophopyrum recombinants and their use in the physical mapping of chromosome 7D. Theor Appl Genet 85:561–567
Kota RS, Gill KS, Gill BS, Endo TR (1993) A cytogenetically based physical map of chromosome 1B in common wheat. Genome 36:548–554
Lagudah ES, Appels R, Brown AHD, McNeil D (1991) The molecular-genetic analysis of Triticum tauschii, the D-genome donor to hexaploid wheat. Genome 34:375–386
Lehfer H, Busch W, Martin R, Herrmann RG (1993) Localization of the B-hordein locus on barley chromosomes using fluorescence in situ hybridization. Chromosoma 102:428–432
Leitch IJ, Leitch AR, Heslopp-Harrison (1991) Physical mapping of plant DNA sequences by simultaneous in situ hybridization of two differently labelled fluorescent probes. Genome 34:329–333
Linde-Laursen I (1979) Giemsa C-banding of barley chromosomes III. Segregation and linkage of C bands on chromosomes 3, 6 and 7. Hereditas 91:73–77
Linde-Laursen I (1982) Linkage map of the long arm of barley chromosome 3 using C-bands and marker genes. Heredity 49:27–35
Liu YG, Tsunewaki K (1991) Restriction fragment length polymorphism (RFLP) analysis in wheat. II. Linkage maps of the RFLP sites in common wheat. Jpn J Genet 66:617–633
Liu CJ, Atkinson MD, Chinoy CN, Devos KM, Gale MD (1992) Nonhomoeologous translocations between group 4, 5 and 7 chromosomes within wheat and rye. Theor Appl Genet 83:305–312
Lukaszewski AJ (1992) A comparison of physical distribution of recombination in chromosome 1R in diploid rye and in hexaploid Triticale. Theor Appl Genet 83:1048–1053
Lukaszewski AJ, Curtis CA (1993) Physical distribution of recombination in B-genome chromosomes of tetraploid wheat. Theor Appl Genet 86:121–127
McIntosh RA (1988) Catalogue of gene symbols for wheat. In: Miller TE, Koebner RMD (eds) Proc 7th Int Wheat Genet Symp, Institute of Plant Science Research Cambridge, UK, pp 1225–1323
Naranjo T, Roca A, Goicoechea PG, Giraldez R (1987) Arm homoeology of wheat and rye chromosomes. In: Miller TC, Koebner RMD (eds) Proc 7th Int Wheat Genet Symp, Institute of Plant Science Research Cambridge. UK, pp 115–120
Slocum MK, Figdore SS, Kennard WC, Suzuki JY, Osborn TC (1990) Linkage arrangement of restriction fragment length polymorphism loci in Brassica oleracea. Theor Appl Genet 80:57–64
Tanksley SD (1988) Molecular mapping of plant chromosomes. Stadler Genet Syrup 18:157–173
Werner JE, Endo TR, Gill BS (1992) Towards a cytogenetically based physical map of the wheat genome. Proc Natl Acad Sci USA 89:11307–11311
Author information
Authors and Affiliations
Additional information
Communicated by R. Herrmann
Rights and permissions
About this article
Cite this article
Hohmann, U., Endo, T.R., Gill, K.S. et al. Comparison of genetic and physical maps of group 7 chromosomes from Triticum aestivum L.. Molec. Gen. Genet. 245, 644–653 (1994). https://doi.org/10.1007/BF00282228
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00282228