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MicroRNA-219-5p exerts tumor suppressor function by targeting ROBO1 in glioblastoma

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Tumor Biology

Abstract

Previous studies have shown that miR-219-5p is dysregulated and exerts tumor-suppressive effects in cancer development and progression. However, the molecular function and mechanism of miR-219-5p in glioblastoma growth and invasion are still unclear. In the present study, we show that miR-219-5p was downregulated in a panel of glioma tissues with different grades and in all the human glioma cell lines examined. Ectopic expression of miR-219-5p inhibited proliferation and invasion and induced apoptosis in vitro, and xenograft formation in vivo. ROBO1 was found to be a direct target of miR-219-5p, and when overexpressed in miR-219-5p-expressing glioma cells, was able to restore proliferative and invasive ability. Finally, in vivo investigation confirmed that miR-219-5p was a tumor suppressor that regulated ROBO1 expression. Taken together, these studies demonstrate that miR-219-5p inhibited cancer cell growth and invasion by direct targeting ROBO1, implicating miR-219-5p as an attractive candidate for cancer therapy.

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Authors and Affiliations

  1. Department of Neurology, Second Affiliated Hospital, Dalian Medical University, No. 467 Zhongshan Road, Dalian, Liaoning, 116027, China

    Yongmei Jiang, Lin Yin, Huirong Jing & Hui Zhang

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  1. Yongmei Jiang
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  2. Lin Yin
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  3. Huirong Jing
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  4. Hui Zhang
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Correspondence to Hui Zhang.

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Jiang, Y., Yin, L., Jing, H. et al. MicroRNA-219-5p exerts tumor suppressor function by targeting ROBO1 in glioblastoma. Tumor Biol. 36, 8943–8951 (2015). https://doi.org/10.1007/s13277-015-3651-4

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  • DOI: https://doi.org/10.1007/s13277-015-3651-4

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