Abstract
Background
Megalobrama is economically one of the most important freshwater fish genera in China. However, phylogenetic relationships among M. amblycephala, M. skolkovii, M. hoffmanni and M. pellegrini remain unresolved.
Objective
To explore the genetic diversity and phylogenetic relationship of Megalobrama populations belonging to all four species.
Methods
The concatenated sequences of mitochondrial cytochrome b (Cytb) and control region (CR) were used to analyze the genetic variation, genetic differentiation and population expansion of 15 Megalobrama populations.
Results
The study showed that haplotype diversity and nucleotide diversity of M. hoffmanni and M. skolkovii were high, and that M. hoffmanni was the most genetically divergent of the four species. Haplotype network analysis revealed that M. hoffmanni and M. amblycephala formed a monophyletic group each, while M. skolkovii and M. pellegrini clustered together. There was a high genetic differentiation among the four Megalobrama species, and genetic distance among populations was not affected by geographical distance. Additionally, the results indicated that there was gene flow between the Liangzi Lake (LZL) population and Jinsha River Reservoir (JS) population. Also, Zhaoqing (ZQ) population of M. hoffmanni might have experienced a population expansion.
Conclusion
Our study verifies genetic diversity and differentiation of Megalobrama populations, and these findings will represent a significant contribution to the conservation and utilization of germplasm resources of Megalobrama.
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (No. 31772901) and the Modern Agriculture Industry Technology System Construction Projects of China entitled Staple Freshwater Fishes Industry Technology System (No. CARS-45-08).
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W.M.W. conceived the study and designed the project. J.C. contributed to collecting samples, analyzed the data, and wrote the manuscript. W.M.W. modified the manuscript. All authors read and approved the final manuscript.
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Chen, J., Wang, W. Genetic diversity and genetic differentiation of Megalobrama populations inferred by mitochondrial markers. Genes Genom 43, 1119–1132 (2021). https://doi.org/10.1007/s13258-021-01126-8
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DOI: https://doi.org/10.1007/s13258-021-01126-8