Skip to main content
SpringerLink
Log in

Isolation and characterization of polymorphic microsatellite markers from the Korean common squid Todarodes pacificus (Ommastrephidae)

  • Research Article
  • Published:
Genes & Genomics Aims and scope Submit manuscript

Abstract

The common squid, Todarodes pacificus, is an important fisheries species, but fluctuations in its abundance and its reproductive biology are poorly understood. To enable sustainable management of this resource, 21 novel polymorphic microsatellite loci were isolated using an enrichment method based on the magnetic/biotin capture of microsatellite sequences from a size-selected genomic library. To characterize each locus, 48 individuals from a natural T. pacificus population from the western coastal waters of Korea were genotyped. All of the microsatellite loci were highly polymorphic with 10–32 alleles per locus. The observed and expected heterozygosities ranged from 0.417 to 0.917 and 0.791 to 0.965, respectively. The high variability revealed in this study suggests that the microsatellite markers could be a useful tool for future conservation genetics studies and for the sustainable exploitation of this resource.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • An HS, Park JY (2005) Ten new highly polymorphic microsatellite loci in the blood clam Scapharca broughtonii. Mol Ecol Notes 5:896–898

    Article  CAS  Google Scholar 

  • An HS, Kim M-J, Hong SW (2008) Genetic diversity of rock bream Oplegnathus fasciatus in Southern Korea. Genes Genom 30:451–459

    CAS  Google Scholar 

  • An HS, Lee JW, Dong CM (2012) Population genetic structure of Korean pen shell (Atrina pectinata) in Korea inferred from microsatellite marker analysis. Genes Genom 34:681–688

    Article  Google Scholar 

  • Asahida T, Kobayashi T, Saitoh K, Nakayama I (1996) Tissue preservation and total DNA extraction from fish stored at ambient temperature using buffers containing high concentrations of urea. Fish Sci Tokyo 62:727–730

    Google Scholar 

  • Callen DF, Thompson AD, Shen Y, Phillips HA, Richards RI, Mulley JC, Sutherland GR (1993) Incidence and origin of “null” alleles in the (AC)n microsatellite markers. Am J Hum Genet 52:922–927

    PubMed Central  CAS  PubMed  Google Scholar 

  • Carleton KL, Streelman JT, Lee BY, Garnhart N, Kidd M, Kocher TD (2002) Rapid isolation of CA microsatellites from the tilapia genome. Anim Genet 33:140–144

    Article  CAS  PubMed  Google Scholar 

  • Excoffer L, Laval G, Schneider S (2005) ARLEQUIN ver 3.0: an integrated software package for population genetics data analysis. Evol Bioinform Online 1:47–50

    Google Scholar 

  • Gardner MG, Cooper SJB, Bull CM, Grant WN (1999) Isolation of microsatellite loci from a social lizard, Egernia stokesii, using a modified enrichment procedure. J Hered 90:301–304

    Article  CAS  Google Scholar 

  • Gong Y, Choi KH (2008) Fluctuations in abundance of common squid, Todarodes pacificus in the far east. J Environ Biol 29:449–452

    PubMed  Google Scholar 

  • Hamilton MB, Pincus EL, DiFiore A, Fleischer RC (1999) Universal linker and ligation procedures for construction of genomic DNA libraries enriched for microsatellites. Biotechniques 27:500–507

    CAS  PubMed  Google Scholar 

  • Hutchings JA, Fraser DJ (2008) The nature of fisheries- and farming-induced evolution. Mol Ecol 17:294–313

    Article  PubMed  Google Scholar 

  • Iwata Y, Lian CL, Sakurai Y (2008) Development of microsatellite markers in the Japanese common Squid Todarodes pacificus (Ommastrephidae). Mol Ecol 8:466–468

    Article  CAS  Google Scholar 

  • Kalinowski ST, Taper ML, Marshall TC (2007) Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment. Mol Ecol 16:1099–1106

    Article  PubMed  Google Scholar 

  • Kanno M, Li Q, Kijima A (2005) Isolation and characterization of twenty microsatellite loci in Japanese sea cucumber (Stichopus japonicus). Mar Biotechnol 7:179–183

    Article  CAS  PubMed  Google Scholar 

  • Kasahara S (1978) Descriptions of offshore squid angling in the sea of Japan with special reference to the distribution of common squid (Todaodes pacificus STEENSTRUP) and on the techniques for forecasting fishing conditions. Bull Japan Sea Reg Fish Res Lab 29:179–199

    Google Scholar 

  • Kawabata A, Yatsu A, Ueno Y, Suyama S, Kurita Y (2006) Spatial distribution of the Japanese common squid, Todaodes pacificus, during its northward migration in the western north Pacific ocean. Fish Oceanorgr 15:113–124

    Article  Google Scholar 

  • Kim W-J, Shin E-H, Kong HJ, Nam B-H, Kim Y-O, Jung H, An CM (2013) Development of Polymorphic Microsatellite Markers Suitable for Genetic Linkage Mapping of Olive Flounder Paralichthys olivaceus. Fish Aquat Sci 16:303–309

    CAS  Google Scholar 

  • Kiyofuji H, Saitoh S (2004) Use of nighttime visible images to detect Japanese common squid Todaodes pacificus fishing areas and potential migration routes in the sea of Japan. Mar Eol Prog Ser 276:173–186

    Article  Google Scholar 

  • Kubata S, Kawabata A (1996) Mantle length composition of Japanese flying squid, Todaodes pacificus Steenstrup, and fluctuations of the migrant stoke size around the north Sanriku coast of Honshu. Bull Tohoku Nat’l Fish Res Inst 58:119–135

    Google Scholar 

  • Li Q, Park C, Kijima A (2002) Isolation and characterization of microsatellite loci in the Pacific abalone, Haliotis discus hannai. J Shell Res 212:811–815

    Google Scholar 

  • Murata M (1991) Squids. In: Nasu K, Okutani T, Ogura M (eds) Nasu. Seizandoshoten, Tokyo, pp 120–143

    Google Scholar 

  • Reiss H, Hoarau G, Dickey-Collas M, Wolff WJ (2009) Genetic population structure of marine fish: mismatch between biological and fisheries management units. Fish Fish 10:361–395

    Article  Google Scholar 

  • Rice WR (1989) Analyzing tables of statistical tests. Evolution 43:223–225

    Article  Google Scholar 

  • Rousset F (2008) Genepop’007: a complete re-implementation of the genepop software for Windows and Linux. Mol Ecol Resour 8:103–106

    Article  PubMed  Google Scholar 

  • Sakurai Y, Bower JR, Ikeda Y (2003) Reproductive characteristics of the ommastrephid Todaodes pacificus. Fisken Og Havet 12:105–115

    Google Scholar 

  • van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:135

    Google Scholar 

  • Ward RD (2006) The importance of identifying spatial population structure in restocking and stock enhancement programmes. Fish Res 80:9–18

    Article  Google Scholar 

  • Zhan A, Hu J, Hu X, Zhou Z, Hui M, Wang P, Wang M, Bao Z (2009) Fine-scale population genetic structure of zhikong scallop (Chlamys farreri): do local marine currents drive geographical differentiation? Mar Biotechnol 11:223–235

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This work was funded by a grant from the Korean National Fisheries Research and Development Institute (NFRDI), contribution number RP-2014-BT-005. The views expressed here are those of the authors and do not necessarily reflect the views of NFRDI.

Conflict of interest

The authors declare no conflict of interest on this article contents.

Author information

Authors and Affiliations

  1. Biotechnology Research Division, National Fisheries Research and Development Institute, Busan, 619-705, Korea

    Hye Suck An, Eun Mi Kim & Chul Min An

  2. Aquaculture Management Division, National Fisheries Research and Development Institute, Busan, 619-705, Korea

    Jeong In Myeong

Authors
  1. Hye Suck An
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Eun Mi Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jeong In Myeong
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Chul Min An
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hye Suck An.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

An, H.S., Kim, E.M., Myeong, J.I. et al. Isolation and characterization of polymorphic microsatellite markers from the Korean common squid Todarodes pacificus (Ommastrephidae). Genes Genom 36, 727–732 (2014). https://doi.org/10.1007/s13258-014-0206-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13258-014-0206-9

Keywords

Search

Navigation