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Molecular characterization of a heat inducible rice gene, OsHSP1, and implications for rice thermotolerance

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Abstract

Higher plants have acquired complex molecular mechanisms to withstand heat stress through years of natural evolutionary processes. Although physiological responses to elevated temperatures have been well studied, thermotolerance mechanisms at the molecular level are poorly understood in rice plants. In order to identify the genes involved in the thermotolerance of rice, we used a publicly available microarray dataset and identified a number of heat stress-responsive genes. Herein, we report details of the rice gene OsHSP1, which is upregulated by heat stress. In addition, OsHSP1 is highly expressed when exposed to salt and osmotic treatments but not cold treatment. Sequence analysis indicated that OsHSP1 belongs to the heat shock protein 90 family of genes. The biological function of OsHSP1 was investigated by heterologous overexpression in Arabidopsis. Transgenic Arabidopsis overexpressing the OsHSP1 gene exhibited enhanced thermotolerance but was hypersensitive under salt and osmotic stresses. Subcellular localization analysis indicated that the OsHSP1 protein is predominantly targeted to the cytosol and nucleus under heat stress. The coexpression network showed 39 interactions for the functionally interacting genes of OsHSP1. Taken together, these findings suggest that OsHSP1 is a heat-inducible gene that may play an important role in the thermotolerance of rice.

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Acknowledgments

This research was supported by iPET (Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries), Ministry for Food, Agriculture, Forestry and Fisheries and a grant from the Next-Generation BioGreen 21 Program (Plant Molecular Breeding Center No. PJ009084), Rural Development Administration, Republic of Korea.

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The authors declare no conflict of interest.

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Authors and Affiliations

  1. Plant Genomics Lab., Department of Applied Plant Sciences, Kangwon National University, Chuncheon, 200-713, Republic of Korea

    Jun-Cheol Moon, Deok Jae Ham, Sun-Goo Hwang, Yong Chan Park & Cheol Seong Jang

  2. Agriculture and Life Sciences Research Institute, Kangwon National University, Chuncheon, 200-713, Republic of Korea

    Jun-Cheol Moon

  3. Department of Plant and Environmental New Resources, KyungHee University, Yongin, 446-701, Republic of Korea

    Chanhui Lee

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  1. Jun-Cheol Moon
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  2. Deok Jae Ham
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  3. Sun-Goo Hwang
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  5. Chanhui Lee
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  6. Cheol Seong Jang
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Correspondence to Cheol Seong Jang.

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Moon, JC., Ham, D.J., Hwang, SG. et al. Molecular characterization of a heat inducible rice gene, OsHSP1, and implications for rice thermotolerance. Genes Genom 36, 151–161 (2014). https://doi.org/10.1007/s13258-013-0152-y

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