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Characterization of a novel variant HMW-glutenin gene from Elymus canadensis

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Abstract

High molecular weight (HMW) glutenin subunits (GS) play a key role in the determination of end-use quality of wheat and other cereal crops. In this study, we report the isolation and characterization of both promoter region and ORF of novel HMW-GS allele 1St1.3 from a perennial Triticeae species, Elymus canadensis. The amino acid (AA) sequences of E. canadensis 1St1.3 were deduced as 434 aa. Its protein primary structure comprises a signal peptide with a conserved N-terminal domain, a central repetitive domain and a C-terminal domain. E. canadensis 1St 1.3 possesses several distinct characteristics which are different from those of wheat HMW-GSs. The N-terminal domains of E. canadensis 1St 1.3 resemble that of y-type subunits, while their C-terminal domains are more similar to x-type subunits. The deletion of 85 bp fragment has been observed in promoter region of 1St 1.3, however which has not interrupted the expression of this gene. Our results indicate that 1St 1.3 is novel HMW-GS variants which will be valuable for enhancing our understanding of structural differentiation and the evolutionary relationship among HMW-GSs in Triticeae species.

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Authors and Affiliations

  1. Triticeae Research Institute, Sichuan Agricultural University, Ya’an, Sichuan, 625014, China

    Qian-Tao Jiang, Yu-Ming Wei, Tao Liu, Ji-Rui Wang & Xiu-Jin Lan

  2. Lethbridge Research Centre, Agriculture and Agri-Food Canada, Lethbridge, T1J 4B1, Canada

    Qian-Tao Jiang & Zhen-Xiang Lu

  3. Key Laboratory of Southwestern Crop Germplasm Utilization, Ministry of Agriculture, Sichuan Agricultural University, Ya’an, Sichuan, 625014, China

    Zhi-En Pu & You-Liang Zhen

Authors
  1. Qian-Tao Jiang
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  2. Yu-Ming Wei
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  3. Zhen-Xiang Lu
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  4. Tao Liu
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  5. Ji-Rui Wang
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  6. Zhi-En Pu
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  7. Xiu-Jin Lan
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  8. You-Liang Zhen
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Correspondence to Zhen-Xiang Lu or You-Liang Zhen.

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Jiang, QT., Wei, YM., Lu, ZX. et al. Characterization of a novel variant HMW-glutenin gene from Elymus canadensis . Genes Genom 32, 361–367 (2010). https://doi.org/10.1007/s13258-010-0028-3

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  • DOI: https://doi.org/10.1007/s13258-010-0028-3

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