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A physiological comparative study of acid tolerance of Lactobacillus plantarum ZDY 2013 and L. plantarum ATCC 8014 at membrane and cytoplasm levels

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Abstract

This study aimed to disclose the acid tolerance mechanism of Lactobacillus plantarum by comparing L. plantarum ZDY 2013 with the type strain L. plantarum ATCC 8014 in terms of cell membrane, energy metabolism, and amino acid metabolism. L. plantarum ZDY 2013 had a superior growth performance under acidic condition with 100-fold higher survival rate than that of L. plantarum ATCC 8014 at pH 2.5. To determine the acid tolerance physiological mechanism, cell integrity was investigated through scanning electron microscopy. The study revealed that L. plantarum ZDY 2013 maintained cell morphology and integrity, which is much better than L. plantarum ATCC 8014 under acid stress. Analysis of energy metabolism showed that, at pH 5.0, L. plantarum ZDY 2013 enhanced the activity of Na+/K+-ATPase and decreased the ratio of NAD+/NADH in comparison with L. plantarum ATCC 8014. Similarly, amino acid metabolism of intracellular arginine, glutamate, and alanine was improved in L. plantarum ZDY 2013. Correspondingly, the activity of arginine deiminase and glutamate decarboxylase of L. plantarum ZDY 2013 increased by 1.2-fold and 1.3-fold compared with L. plantarum ATCC 8014 in acid stress. In summary, it is demonstrated that the special physiological behaviors (integrity of cell membrane, enhanced energy metabolism, increased amino acid and enzyme level) of L. plantarum ZDY 2013 can protect the cells from acid stress.

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Acknowledgments

This project was sponsored by the National Natural Science Foundation of China (NSF31170091, 81760102, 31360377 and 31260363), the Ganpo Talent Engineering 555 Project, the Academic and Technical Leaders Training Program for Major Subjects of Jiangxi Province (P. R. China), the Research Project of Jiangxi Provincial Education Department (GJJ13098; P. R. China), and the Postdoctoral Science Foundation Funded Project of China (M570567).

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Authors and Affiliations

  1. State Key Laboratory of Food Science and Technology, Nanchang University, 235 Nanjing Donglu, Nanchang, Jiangxi, 330047, China

    Yilin Guo, Ximei Tian, Renhui Huang, Xueying Tao & Hua Wei

  2. Food and Nutritional Science, School of Biological Science, The University of Hong Kong, Pokfulam Road, Hong Kong, China

    Nagendra P. Shah

  3. Jiangxi-OAI Joint Research Institute, Nanchang University, 235 Nanjing Donglu, Nanchang, 330047, People’s Republic of China

    Hua Wei & Cuixiang Wan

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  1. Yilin Guo
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  2. Ximei Tian
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  3. Renhui Huang
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  4. Xueying Tao
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  6. Hua Wei
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  7. Cuixiang Wan
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Correspondence to Cuixiang Wan.

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Guo, Y., Tian, X., Huang, R. et al. A physiological comparative study of acid tolerance of Lactobacillus plantarum ZDY 2013 and L. plantarum ATCC 8014 at membrane and cytoplasm levels. Ann Microbiol 67, 669–677 (2017). https://doi.org/10.1007/s13213-017-1295-x

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  • DOI: https://doi.org/10.1007/s13213-017-1295-x

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