Skip to main content
SpringerLink
Log in

Bioconversion of ferulic acid to vanillic acid by Paenibacillus lactis SAMS-2001

  • Original Article
  • Published:
Annals of Microbiology Aims and scope Submit manuscript

Abstract

Ferulic acid is an abundant cinnamic acid derivative found in the plant world and has been utilized by microorganisms to produce value-added compounds such as vanillin and vanillic acid. The isolate SAMS-2001, giving the highest vanillic acid yield, was selected and identified as Paenibacillus lactis based on its 16S rDNA sequence (GenBank accession number KF699133). Vanillic acid was found to accumulate in the minimal medium as the major product along with the transient formation of vanillin by adding yeast (0.05 %) as co-substrate. In vitro conversion of ferulic acid to vanillin and vanillic acid was also studied with the cell extract of Paenibacillus lactis SAMS-2001. This study gives the first evidence for production of vanillic acid (57.3 % molar yield) from ferulic acid within 18 h using Paenibacillus lactis SAMS-2001.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Abdelkafi S, Labat M, Gam ZBA, Lorquin J, Casalot L, Sayadi S (2008) Optimized conditions for the synthesis of vanillic acid under hypersaline conditions by Halomonas elongata DSM 2581T resting cells. World J Microbiol Biotechnol 24(5):675–680

    Article  CAS  Google Scholar 

  • Ashengroph M, Nahvi I, Zarkesh-Esfahani H, Momenbeik F (2012) Novel strain of Bacillus licheniformis SHL1 with potential converting ferulic acid into vanillic acid. Ann Microbiol 62(2):553–558

    Article  CAS  Google Scholar 

  • Breed RS, Murray EGD, Smith NR (1957) Bergey’s manual of determinative bacteriology. The Williams and Wilkins Company, Baltimore

    Google Scholar 

  • Brunati M, Marinelli F, Bertolini C, Gandolfi R, Daffonchio D, Molinari F (2004) Biotransformations of cinnamic and ferulic acid with actinomycetes. Enzym Microb Technol 34(1):3–9

    Article  CAS  Google Scholar 

  • Civolani C, Barghini P, Roncetti AR, Ruzzi M, Schiesser A (2000) Bioconversion of ferulic acid into vanillic acid by means of a vanillate-negative mutant of Pseudomonas fluorescens strain BF13. Appl Environ Microbiol 66(6):2311–2317

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Dey G, Sachan A, Ghosh S, Mitra A (2003) Detection of major phenolic acids from dried mesocarpic husk of mature coconut by thin layer chromatography. Ind Crop Prod 18(2):171–176

    Article  CAS  Google Scholar 

  • Estrada-Alvarado I, Lomascolo A, Navarro D, Delattre M, Asther M, Lesage-Meessen L (2001) Evidence of new biotransformation pathway of p-coumaric acid into p-hydroxy-benzaldehyde in Picnoporus cinnabarinus. Appl Microbiol Biotechnol 57(5–6):725–730

    CAS  PubMed  Google Scholar 

  • Garcia MT, Ventosa A, Mellado E (2005) Catabolic versatility of aromatic compound-degrading halophilic bacteria. FEMS Microbiol Ecol 54(1):97–109

    Article  CAS  PubMed  Google Scholar 

  • Ghosh S, Sachan A, Mitra A (2006) Formation of vanillic acid from ferulic acid by Paecilomyces variotii MTCC 6581. Curr Sci 90(6):825–829

    CAS  Google Scholar 

  • Ghosh S, Sachan A, Sen SK, Mitra A (2007) Microbial transformation of ferulic acid to vanillic acid by Streptomyces sannanensis MTCC 6637. J Ind Microbiol Biotechnol 34(2):131–138

    Article  CAS  PubMed  Google Scholar 

  • Gurujeyalakshmi G, Mahadevan A (1987) Dissimilation of ferulic acid by Bacillus subtilis. Curr Microbiol 16(2):69–73

    Article  CAS  Google Scholar 

  • Hall BG (2013) Building phylogenetic trees from molecular data with MEGA. Mol Biol Evol 30(5):1229–1235

    Article  CAS  PubMed  Google Scholar 

  • Kaur B, Chakraborty D, Kumar B (2013) Phenolic biotransformations during conversion of ferulic acid to vanillin by lactic acid bacteria. Biomed Res Int 590359:1–6

    Google Scholar 

  • Kim SJ, Kim MC, Um JY, Hong SH (2010) The beneficial effect of vanillic acid on ulcerative colitis. Molecules 15(10):7208–7217

    Article  CAS  PubMed  Google Scholar 

  • Longo MA, Sanroman MA (2006) Production of food aroma compounds: microbial and enzymatic methodologies. Food Technol Biotechnol 44(3):335–353

    CAS  Google Scholar 

  • Mariod AA, Adamu HA, Ismail M, Ismail N (2010) Antioxidative effects of stabilized and unstabilized defatted rice bran methanolic extracts on the stability of rice bran oil under accelerated conditions. Grasas y Aceites 61(4):409–415

    Article  CAS  Google Scholar 

  • Muheim A, Lerch K (1999) Towards a high-yield bioconversion of ferulic acid to vanillin. Appl Microbiol Biotechnol 51(4):456–461

    Article  CAS  Google Scholar 

  • Narbad A, Gasson MJ (1998) Metabolism of ferulic acid via vanillin using a novel CoA-dependent pathway in a newly isolated strain of Pseudomonas fluorescens. Microbiology 144(5):1397–1405

    Article  CAS  PubMed  Google Scholar 

  • Oddou J, Stentelaire C, Lesage-Meessen L, Asther M, Ceccaldi BC (1999) Improvement of ferulic acid bioconversion into vanillin by use of high-density cultures of Pycnoporus cinnabarinus. Appl Microbiol Biotechnol 53(1):1–6

    Article  CAS  Google Scholar 

  • Rosazza JPN, Huang Z, Dostal L, Volm T, Rousseau B (1995) Biocatalytic transformation of ferulic acid: an abundant aromatic natural product. J Ind Microbiol 15(6):457–471

    Article  CAS  PubMed  Google Scholar 

  • Sachan A, Ghosh S, Mitra A (2004) An efficient isocratic separation of hydroxycinnamates and their corresponding benzoates from microbial and plant sources by HPLC. Biotechnol Appl Biochem 40(2):197–200

    Article  CAS  PubMed  Google Scholar 

  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4(4):406–425

    CAS  PubMed  Google Scholar 

  • Scheldeman P, Goossens K, Rodriguez-Diaz M, Pil A, Goris J, Herman L, Vos PD, Logan NA, Heyndrickx M (2004) Paenibacillus lactis sp. nov., isolated from raw and heat-treated milk. Int J Syst Evol Microbiol 54:885–891

  • Serra S, Fuganti C, Brenna E (2005) Biocatalytic preparation of natural flavours and fragrances. Trends Biotechnol 23(4):193–198

    Article  CAS  PubMed  Google Scholar 

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25(24):4876–4882

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Topakas E, Kalogeris E, Kekos D, Macris BJ, Christakopolos P (2003) Bioconversion of ferulic acid into vanillic acid by the thermophilic fungus Sporotrichum thermophile. Lebensm Wiss Technol 36(6):561–565

    Article  CAS  Google Scholar 

  • Vyrides I, Agathangelou M, Dimitriou R, Souroullas K, Salamex A, Ioannou A, Koutinas M (2015) Novel Halomonas sp. B15 isolated from Larnaca Salt Lake in Cyprus that generates vanillin and vanillic acid from ferulic acid. World J Microbiol Biotechnol 31(8):1291–1296

    Article  CAS  PubMed  Google Scholar 

  • Walton NJ, Narbad A, Faulds CB, Williamson G (2000) Novel approaches to the biosynthesis of vanillin. Curr Opin Biotechnol 11(5):490–496

    Article  CAS  PubMed  Google Scholar 

  • Zamzuri NA, Abd-Aziz S, Rahim RA, Phang LY, Alitheen NB, Maeda T (2014) A rapid colorimetric screening method for vanillic acid and vanillin-producing bacterial strains. J Appl Microbiol 116:903–910

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This study was financially supported by University Grant Commission [37-115/2009 (SR)], New Delhi. Shashank Mishra gratefully acknowledges the Department of Bio-Engineering, Birla Institute of Technology, Mesra, for providing infrastructure facilities. We also acknowledge Dr. Sanjaya Swain (Research Officer), Central Instrumentation Facility, BIT, Mesra for providing analytical facilities.

Author information

Authors and Affiliations

  1. Department of Bio-Engineering, Birla Institute of Technology, Mesra, Ranchi, Jharkhand, 835 215, India

    Shashank Mishra, Meenakshi Kullu, Ashish Sachan & Shashwati Ghosh Sachan

  2. Institute of Engineering & Technology, Sitapur Road, Lucknow, Uttar Pradesh, 226 021, India

    Ambarish Sharan Vidyarthi

Authors
  1. Shashank Mishra
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Meenakshi Kullu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ashish Sachan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ambarish Sharan Vidyarthi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Shashwati Ghosh Sachan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Shashwati Ghosh Sachan.

Ethics declarations

This article does not contain any studies with human participants or animals performed by any of the authors.

Conflict of interest

The authors declare that there is no conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Mishra, S., Kullu, M., Sachan, A. et al. Bioconversion of ferulic acid to vanillic acid by Paenibacillus lactis SAMS-2001. Ann Microbiol 66, 875–882 (2016). https://doi.org/10.1007/s13213-015-1175-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13213-015-1175-1

Keywords

Search

Navigation