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Characterisation and growth kinetics studies of caffeine-degrading bacterium Leifsonia sp. strain SIU

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Abstract

Caffeine is an important naturally occurring compound that can be degraded by bacteria. Excessive caffeine consumption is known to have some adverse effects. We isolated a new bacterium from agriculture soil. The bacterium was tested for its ability to utilise caffeine as the sole carbon and nitrogen source. The isolate was Gram-negative and was identified as Leifsonia sp. strain SIU based on 16S rRNA gene sequencing. It showed 97.16 % of 0.3 g/L caffeine degradation in 48 h when caffeine was used as a sole carbon and nitrogen source. The bacterial growth and degradation at 0.3 g/L caffeine concentration occurred optimally, using 5 g/L sucrose, 0.4 g/L ammonium chloride, at a temperature between 25 and 30 °C and pH of 6.0–7.0. The Luong model best describes the kinetics of the strain growth. The values for the maximum specific growth rate (μ max ), the Monod half saturation constant (K S ), the maximum substrate inhibitory concentration and n are 0.049 h−1, 0.0021 mg/L, 25.0 g/L and 1.562, respectively. These bacterial features make it an ultimate means for caffeine bioremediation. This is the first report of caffeine degradation by Leifsonia sp. strain SIU.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

    Salihu Ibrahim, Mohd Yunus Shukor, Mohd Arif Syed & Siti Aqlima Ahmad

  2. Department of Biochemistry, Faculty of Biomedical Sciences, Bayero University, PMB 3011, Kano, Nigeria

    Salihu Ibrahim

  3. Department of Environmental Sciences, Faculty of Environmental Studies, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

    Wan Lutfi Wan Johari

  4. Environmental Forensics Research Centre, Faculty of Environmental Studies, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

    Wan Lutfi Wan Johari

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  1. Salihu Ibrahim
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  2. Mohd Yunus Shukor
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Correspondence to Siti Aqlima Ahmad.

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Ibrahim, S., Shukor, M.Y., Syed, M.A. et al. Characterisation and growth kinetics studies of caffeine-degrading bacterium Leifsonia sp. strain SIU. Ann Microbiol 66, 289–298 (2016). https://doi.org/10.1007/s13213-015-1108-z

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  • DOI: https://doi.org/10.1007/s13213-015-1108-z

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