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Microbial Degradation of Caffeine Using Himalayan Psychrotolerant Pseudomonas sp.GBPI_Hb5 (MCC 3295)

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Abstract

Caffeine, a xenobiotic compound, is continuously released into the environment. Fifteen psychrotolerant bacterial strains, isolated from the Indian Himalayan region, were screened for their caffeine degradation capacity. The medium for the growth of bacteria was optimized using Box-Behnken method. Among these bacteria, Pseudomonassp. (GBPI_Hb5), showing the best response, was further used for caffeine degradation in batch mode. The culture medium, having caffeine as a sole source of carbon, was used for analyzing the effect of pH, agitation speed, temperature, inoculum volume, and caffeine concentration on bacterial growth and its caffeine degradation potential. The bacterium GBPI_Hb5 showed approx. 93% caffeine degradation up to 96 h under controlled conditions. The compounds produced during the degradation of caffeine were also studied. The study is likely to have implications in the bioremediation of caffeine from polluted environments.

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Acknowledgements

Authors are grateful to Director GBP-NIHE for extending the facilities and Department of Science and Technology-Water Technology Initiative (DST-WTI) for financial support.

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Authors and Affiliations

  1. Centre for Land and Water Resource Management, G. B. Pant National Institute of Himalayan Environment, Kosi-Katarmal, Almora, Uttarakhand, 263643, India

    Pooja Thathola & Vasudha Agnihotri

  2. Department of Biotechnology, Graphic Era University, Bell Road, Clement Town, Dehradun, Uttarakhand, 248002, India

    Anita Pandey

Authors
  1. Pooja Thathola
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  2. Vasudha Agnihotri
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  3. Anita Pandey
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Contributions

Laboratory work and original draft preparation was done by PT and VA. The conceptualization, formal analysis, funding, acquisition, resources, software, supervision, and review were done by VA. Final editing by VA and AP. Microbial strains are provided by AP.

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Correspondence to Vasudha Agnihotri.

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Thathola, P., Agnihotri, V. & Pandey, A. Microbial Degradation of Caffeine Using Himalayan Psychrotolerant Pseudomonas sp.GBPI_Hb5 (MCC 3295). Curr Microbiol 78, 3924–3935 (2021). https://doi.org/10.1007/s00284-021-02644-0

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