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Impact of continuous Salvia miltiorrhiza cropping on rhizosphere actinomycetes and fungi communities

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Abstract

Salvia miltiorrhiza Bunge (SMB) is an important herb that has been used in traditional Chinese medicine for centuries. Continuous SMB cropping can result in significant losses of yield and quality of the plant. The purpose of this study was to examine the variations in community structure of soil actinomycetes and fungi under continuous cropping of SMB in Sichuan, China. Four soil samples were analyzed by polymerase chain reaction and denaturing gradient gel electrophoresis (PCR-DGGE). The microbial diversity indices (Shannon-Wiener index, richness, and evenness) of the soil actinomycetes decreased along with continuous SMB cropping and rebound at the 3rd year, while that of soil fungi declined. Cluster analysis showed that the similarity between uncultivated soils and cropping soils declined with subsequent cropping periods. Homology search of sequences recovered from the DGGE bands showed that the actinomycete community in the studied soils was dominated by Actinobacteria, while dominant species of the fungal community varied remarkably, including Pleospora, Psathyrella, Pseudozyma, Rhizoctonia, Trichophaea and unclassified groups. Overall, these findings demonstrate that continuous SMB cropping has a significant impact on soil actinomycete and fungal communities.

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Acknowledgments

We are grateful to the anonymous reviewers for their critical reading of the manuscript. This research was funded by a Key Project of Shenzhen Emerging Industries (No. JC201104210118A) and National Natural Science Foundation of China (No.81130070).

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Authors and Affiliations

  1. School of Environment and Energy, Peking University-Shenzhen Graduate School, Shenzhen, 518055, China

    Jie Tang, Zhiquan Xue, Maurycy Daroch & Jiong Ma

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  1. Jie Tang
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  2. Zhiquan Xue
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Correspondence to Jiong Ma.

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Jie Tang and Zhiquan Xue contributed equally to this work.

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Tang, J., Xue, Z., Daroch, M. et al. Impact of continuous Salvia miltiorrhiza cropping on rhizosphere actinomycetes and fungi communities. Ann Microbiol 65, 1267–1275 (2015). https://doi.org/10.1007/s13213-014-0964-2

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  • DOI: https://doi.org/10.1007/s13213-014-0964-2

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