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Fermentation characteristics and hypoglycemic activity of an exopolysaccharide produced by submerged culture of Stropharia rugosoannulata #2

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Abstract

We have investigated the mycelial growth and extracellular polysaccharide (EPS) production of Stropharia rugosoannulata #2 under optimal culture conditions in a 5-L stirred-tank fermenter. Maximum biomass growth (16.35 g/L) was achieved after 5 days of cultivation, whereas EPS reached its maximum level (10.83 g/L) after 8 days. The morphological parameters (i.e., mean diameter, circularity, roughness, compactness) of the fungal pellets and broth viscosity were also characterized. The compactness of the pellets was determined to be significantly and positively correlated with EPS content. The hypoglycemic effect of the polysaccharide, investigated in streptozotocin-induced diabetic rats, included decreases in the plasma concentrations of glucose (37 %), total cholesterol (26 %), and triacylglycerol (24 %) and decreased aspartate aminotransferase activity (20 %). The results indicate the potential of this polysaccharide to prevent hyperglycemia in diabetic patients.

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Authors and Affiliations

  1. College of Life Sciences, Henan Normal University, Henan, 450002, China

    Xinhui Zhai & Aijing Zhao

  2. College of Food and Biological Engineering, Zhengzhou University of Light Industry, Henan, 450002, China

    Lujing Geng & Chunping Xu

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  1. Xinhui Zhai
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  2. Aijing Zhao
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  3. Lujing Geng
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  4. Chunping Xu
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Correspondence to Chunping Xu.

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Zhai, X., Zhao, A., Geng, L. et al. Fermentation characteristics and hypoglycemic activity of an exopolysaccharide produced by submerged culture of Stropharia rugosoannulata #2. Ann Microbiol 63, 1013–1020 (2013). https://doi.org/10.1007/s13213-012-0555-z

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  • DOI: https://doi.org/10.1007/s13213-012-0555-z

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