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Production of extracellular polysaccharides by submerged mycelial culture of Laetiporus sulphureus var. miniatus and their insulinotropic properties

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Abstract

In the present study, optimum culture conditions for the production of extracellular polysaccharides (EPS) in submerged culture of an edible mushroom, Laetiporus sulphureus var. miniatus and their stimulatory effects on insulinoma cell (RINm5F) proliferation and insulin secretion were investigated. The maximum mycelial growth (4.1 g l−1) and EPS production (0.6 g l−1) in submerged flask culture were achieved in a medium containing 30 g l−1 maltose, 2 g l−1 soy peptone, and 2 mM MnSO4·5H2O at an initial pH 2.0 and temperature 25°C. In the stirred-tank fermenter under optimized medium, the concentrations of mycelial biomass and EPS reached a maximum level of 8.1 and 3.9 g l−1, respectively. Interestingly, supplementation of deep sea water (DSW) into the culture medium significantly increased both mycelial biomass and EPS production by 4- and 6.7-fold at 70% (v/v) DSW medium, respectively. The EPS were proved to be glucose-rich polysaccharides and were able to increase proliferation and insulin secretary function of rat insulinoma RINm5F cells, in a dose-dependent manner. In addition, EPS also strikingly reduced the streptozotocin-induced apoptosis in RINm5F cells indicating the mode of the cytoprotective role of EPS on RINm5F cells.

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Acknowledgement

This research was supported by the Korea Institute of Industrial Technology Evaluation and Planning Grant funded by Ministry of Commerce, Industry, and Energy of Korea (2006, Regional Innovation System).

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Authors and Affiliations

  1. Department of Biotechnology, Daegu University, Kyungsan, Kyungbuk, 712-714, South Korea

    Hee Sun Hwang, Sung Hak Lee, Yu Mi Baek, Sang Woo Kim & Jong Won Yun

  2. Department of Biotechnology, Dong-A University, Busan, 604-714, South Korea

    Yong Kee Jeong

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  1. Hee Sun Hwang
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  2. Sung Hak Lee
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Correspondence to Jong Won Yun.

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Hwang, H.S., Lee, S.H., Baek, Y.M. et al. Production of extracellular polysaccharides by submerged mycelial culture of Laetiporus sulphureus var. miniatus and their insulinotropic properties. Appl Microbiol Biotechnol 78, 419–429 (2008). https://doi.org/10.1007/s00253-007-1329-6

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