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Immobilization of Rhizopus oryzae in a modified polyvinyl alcohol gel for L(+)-lactic acid production

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Abstract

The production of L(+)-lactic acid (LA) by Rhizopus oryzae immobilized in polyvinyl alcohol (PVA) was investigated. To decrease diffusional resistance, we modified the PVA gel through the addition of sodium alginate and phosphate esterification. The production of L(+)-LA improved notably in the immobilized Rhizopus oryzae. Maximum L(+)-LA production (106.27 g/L), with a yield of 73.1 % and rate of 2.95 g/L·h, was obtained at a temperature of 38 °C, 6 % PVA, and 0.8 % sodium alginate. The immobilized R. oryzae was stable in 14 serial-batch cultures using non-growth medium. The immobilized beads also displayed good tolerance to low temperature and long-term storage at 4 °C with the preservation of biochemical properties.

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Abbreviations

LA:

Lactic acid

PLA:

Polylactic acid

PVA:

Polyvinyl alcohol

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Acknowledgments

This work was supported by the National High Technology Research and Development Program of China (No. 2011AA02A202) and the Nature Science Foundation of Anhui province (No. 1208085QA06).

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Authors and Affiliations

  1. Key Lab of Ion Beam Bioengineering, Chinese Academy of Science, P. O. Box 1126, Hefei, Anhui, 230031, People’s Republic China

    Peng Wang, Zhen Chen, Juan Li, Li Wang, Guohong Gong, Genhai Zhao, Hui Liu & Zhiming Zheng

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  1. Peng Wang
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  2. Zhen Chen
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  3. Juan Li
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  4. Li Wang
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  5. Guohong Gong
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  6. Genhai Zhao
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  7. Hui Liu
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  8. Zhiming Zheng
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Corresponding author

Correspondence to Zhiming Zheng.

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Peng Wang and Zhen Chen contributed equally to this work.

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Wang, P., Chen, Z., Li, J. et al. Immobilization of Rhizopus oryzae in a modified polyvinyl alcohol gel for L(+)-lactic acid production. Ann Microbiol 63, 957–964 (2013). https://doi.org/10.1007/s13213-012-0549-x

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