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Glucose detection using 4-mercaptophenyl boronic acid-incorporated silver nanoparticles-embedded silica-coated graphene oxide as a SERS substrate

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Abstract

In this work, 4-mercaptophenyl boronic acid (4-MPBA) was self-assembled on the surface of silver nanoparticle-embedded silica-coated graphene oxide (GO@SiO2@Ag NPs@MPBA) to detect glucose by surface enhanced Raman scattering (SERS). The SERS intensity of 4-MPBA on the GO@SiO2@Ag NPs was 2.2-fold greater than that of GO@Ag NPs. Moreover, silica-coated GO exhibited lower background signals compared to GO. The SERS intensity of GO@SiO2@Ag NPs@MPBA peaked at 1 mM 4-MPBA. pH-dependent behavior of 4-MPBA on the GO@SiO2 @Ag NPs was investigated; the highest SERS signal intensity was detected at pH 3. The binding of glucose to 4-MPBA-incorporated GO@SiO2@Ag NPs increased the SERS signals at both 1,072 and 1,588 cm-1. The linear range was estimated from 2 to 20 mM glucose. These results provide insight into detection of glucose and the development of SERS-based biosensors using graphene oxide.

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Authors and Affiliations

  1. Department of Bioscience and Biotechnology, Konkuk University, Seoul, 05029, Republic of Korea

    Xuan-Hung Pham, Seongbo Shim, Tae-Han Kim, Eunil Hahm, Hyung-Mo Kim, Won-Yeop Rho & Bong-Hyun Jun

  2. Department of Chemistry Education, Seoul National University, Seoul, 08826, Republic of Korea

    Dae Hong Jeong

  3. School of Chemical and Biological Engineering, Seoul National University, Seoul, 08826, Republic of Korea

    Yoon-Sik Lee

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  1. Xuan-Hung Pham
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  2. Seongbo Shim
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Pham, XH., Shim, S., Kim, TH. et al. Glucose detection using 4-mercaptophenyl boronic acid-incorporated silver nanoparticles-embedded silica-coated graphene oxide as a SERS substrate. BioChip J 11, 46–56 (2017). https://doi.org/10.1007/s13206-016-1107-6

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