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Probiotic potential of Weissella paramesenteroides MYPS5.1 isolated from customary dairy products and its therapeutic application

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Abstract

Probiotics are viable microorganisms that confer general health benefits to the host when consumed in an adequate concentration. Probiotics may also possess strain-specific therapeutic properties and therefore finding novel strains with probiotic properties is becoming increasingly important. The present study has focused on the isolation of probiotic bacteria from dairy products which possessed potential therapeutic properties. Of the 79 strains isolated, eight were selected for further studies based on a number of traits including biofilm formation, deoxyribonuclease (DNase) activity, agglutination activity, auto-aggregation activity, antibiotic resistance, and antagonistic activity. Strain MYPS5.1 was selected from the eight isolates as the best potential probiotic candidate strain and was subsequently identified as Weissella paramesenteroides by 16S rDNA gene sequencing and sequence analysis. W. paramesenteroides strain MYPS5.1 was resistant to a number of antibiotics and the strain produced a high concentration of exopolysaccharide (EPS) (380.42 mg/L). The functional groups C–H, C = C, N = N, N–H, and C–O in the EPS were identified by using Fourier transform infrared (FTIR) spectroscopy. Computational studies showed that it interacted with cyclin-dependent kinase (CDK), a molecule which is thought to play a role in cancer pathogenesis (REF). Collectively, these results suggest that Weissella paramesenteroides MYPS5.1 is a potential probiotic strain with potential therapeutic properties.

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Acknowledgements

PS acknowledges the Lab Infrastructure grant by BHU, Varanasi (F(C)/XVIII-Spl.Fund/Misc/Infrastructure/Instt.Sc/2019-2020/10290) and BTISNet-Sub-Distributed Information Centre, funded by DBT, Govt. of India at the School of Biotechnology, Banaras Hindu University, Varanasi, India. PS acknowledges the infrastructural support from Department of Science and Technology, New Delhi, Govt. of India, through FIST grant (Grant No. 1196 SR/FST/LS-I/2017/4) and Department of Biotechnology, Government of India (Grant no. BT/PR27437/BCE/8/1433/2018). MY acknowledges the support as the University Research Scholarship (URS) by M.D. University, Rohtak, India.

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  1. Pratyoosh Shukla

    Present address: School of Biotechnology, Institute of Science, Banaras Hindu University, Varanasi, 221005, India

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  1. Enzyme Technology and Protein Bioinformatics Laboratory, Department of Microbiology, Maharshi Dayanand University, Rohtak, 124001, Haryana, India

    Monika Yadav,  Sunita & Pratyoosh Shukla

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MY did the experiments and wrote the first draft of the manuscript along with editing contribution from SV. The manuscript was edited and revised by PS. All the authors approved for its submission.

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Yadav, M., Sunita & Shukla, P. Probiotic potential of Weissella paramesenteroides MYPS5.1 isolated from customary dairy products and its therapeutic application. 3 Biotech 12, 9 (2022). https://doi.org/10.1007/s13205-021-03074-2

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