Abstract
Bacillus spp. have been widely reported with the ability to control plant diseases. In this work, we analyzed the whole genome of LJBS06, which was isolated from grapevine rhizosphere soil. In view of physiological and biochemical characteristics, genome data, and phylogenetic analysis of 16S rRNA, LJBS06 was affiliated with Bacillus stercoris. LJBS06 showed antagonistic activities against a variety of plant pathogens. The inhibition rate of Magnaporthe oryzae was up to 75.05% and the inhibition rates of Colletotrichum gloeosporioides, Coniothyrium diplodiella, and Botrytis cinerea were all above 50% in the plate assays. The genome of LJBS06 had a 4,154,362-bp circular chromosome, with an average GC content of 43.96%, containing an 82,935-bp plasmid with a GC content of 35.18%. The circular chromosome of LJBS06 contained 4231 protein-coding genes, 30 rRNA genes, and 87 tRNA genes, including genes related to the synthesis of plant defense-related enzymes and the promotion of plant growth. Meanwhile, 11 gene clusters involved in biosynthesis of secondary metabolites were present in the genome of LJBS06. In conclusion, our findings indicated that LJBS06 strain had the necessary genetic machinery to control plant pathogens and provided insights for future studies of the biocontrol mechanisms of B. stercoris LJBS06.
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The GenBank accession number for the 16S rRNA gene of Bacillus stercoris LJBS06 is MW940707. The complete sequence of the chromosome of the strain B. stercoris LJBS06 and the sequence of the plasmid have been deposited in GenBank database under the accession number CP070483 and CP070484, respectively. The strain B. stercoris LJBS06 was publicly available from China General Microbiological Culture Collection Center (CGMCC) under the accession number CGMCC No. 21263. General characteristics of B. stercoris LJBS06, effect of LJBS06 on the mycelia of C. gloeosporioides, plate assays for the activities of extracellular enzymes, genome statistics, COG categories of the chromosome and the plasmid of B. stercoris LJBS06, inhibition diameter of B. stercoris LJBS06 against 12 pathogens, genes related to defense-related enzymes in the LJBS06 genome, and genes attributed to plant growth promoting traits in the LJBS06 genome are available as Supplementary Materials.
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This work was supported by Shanghai Municipal Commission for Science and Technology (18391900400), National Key R & D Program of China (2019YFD1002501), and China Postdoctoral Science Foundation (18Z102060107 and 20Z102060031).
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by BW, HP, WW, BY, YC, FX, YP, PF. The first draft of the manuscript was written by BW and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Wang, B., Peng, H., Wu, W. et al. Genomic insights into biocontrol potential of Bacillus stercoris LJBS06. 3 Biotech 11, 458 (2021). https://doi.org/10.1007/s13205-021-03000-6
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DOI: https://doi.org/10.1007/s13205-021-03000-6