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Protease and γ-glutamyltranspeptidase activities of bacillus spp. isolated from rice straw

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Abstract

Bacillus strains were isolated from the rice straw of 7 districts in Korea, and almost all were identified as either B. amyloliquefaciens, B. pumilus, or B. subtilis. The correlation between the protease activity and γ-glutamyltranspeptidase activity produced by the identified Bacillus spp. was positively correlated (R2rF0.747). Statistically, the B. subtilis species might be the most potent producer of protease and γ-glutamyltranspeptidase of 3 identified Bacillus strains.

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References

  1. Schallmey M, Singh A, Ward OP. Development in the use of Bacillus species for industrial production. Can. J. Microbiol. 50: 1–17 (2004)

    Article  CAS  Google Scholar 

  2. Oh JH, Lee BJ, Paik HR, Jung SC, Baik KS, Choi SK. Isolation of bacteria from cheonggukjang prepared by rice straw and identification of protease secreted. J. Life Sci. 19: 397–402 (2009)

    Article  Google Scholar 

  3. Kang SE, Rhee JH, Park C, Sung MH, Lee I. Distribution of poly-γ-glutamate (γ-PGA) producers in Korean fermented foods, cheonggukjang, doenjang, and gochujang. Food Sci. Biotechnol. 14: 704–708 (2005)

    CAS  Google Scholar 

  4. Park C, Choi YH, Shin HJ, Poo H, Song JJ, Kim CJ, Sung MH. Effect of high-molecular-weight poly-γ-glutamic acid from Bacillus subtilis (cheonggukjang) on Ca solubility and intestinal absorption. J. Microbiol. Biotechn. 15: 855–858 (2005)

    CAS  Google Scholar 

  5. Meerak J, Lida H, Watanabe Y, Miyashita M, Sato H, Nakagawa Y, Tahara Y. Phylogeny of γ-polyglutamic acid-producing Bacillus strains isolated from fermented soybean foods manufactured in Asian countries. J. Gen. Appl. Microbiol. 53: 315–323 (2007)

    Article  CAS  Google Scholar 

  6. Lee SL. Effect of biopolymer produced by mixed Bacillus strains on the antioxidant activity and inhibitory activities of tyrosinase and elastase. J. Korean Soc. Cosm. 15: 562–570 (2009)

    Google Scholar 

  7. Zhao XQ, Park KH, Jin YY, Lee IH, Yang YY, Suh JW. Isolation and characterization of a new γ-polyglutamic acid producer, Bacillus mesentericus MJM1, from Korean domestic cheonggukjang bean paste. J. Microbiol. Biotechn. 15: 59–65 (2005)

    CAS  Google Scholar 

  8. Sneath PHA, Mair NS, Sharpe ME, Holt JG. Endospore forming gram positive rods and cocci. pp. 1104–1139. In: Bergey’s Manual of Systematic Bacteriology. Williams & Wilkins, Baltimore, MD, USA ( 1986)

    Google Scholar 

  9. Prestidge L, Gage V, Spizizen J. Protease activities during the course of sporulaion in Bacillus subtilis. J. Bacteriol. 107: 815–823 (1971)

    CAS  Google Scholar 

  10. Kwon HY, Kim YS, Kwon GS, Kwon CS, Sohn HY. Isolation of immune-stimulating strain Bacillus pumilus JB-1 from cheonggukjang and fermentational characteristics of JB-1. Korean J. Microbiol. Biotechnol. 32: 291–296 (2004)

    CAS  Google Scholar 

  11. Ahn YS, Kim YS, Shin DH. Isolation, identification, and fermentation characteristics of Bacillus sp. with high protease activity from traditional cheonggukjang. Korean J. Food Sci. Technol. 38: 82–87 (2006)

    Google Scholar 

  12. Amoa-Awua WK, Terlabie NN, Sakyi-Dawson E. Screening of 42 Bacillus isolates for ability to ferment soybean into dawadawa. Int. J. Food Microbiol. 106: 343–347 (2006)

    Article  CAS  Google Scholar 

  13. Lee NK, Jeon EH, Lee HJ, Cho IJ, Hahm YT. Isolation, identification, and characterization of Bacillus spp. from the traditionally fermented cheonggukjangs in the Gyeonggi and the Gangwon provinces. J. Korean Soc. Appl. Biol. Chem. 49: 276–280 (2006)

    CAS  Google Scholar 

  14. Choi KK, Cui CB, Ham SS, Lee DS. Isolation, identification, and growth characteristics of main strain related to meju fermentation. J. Korean Soc. Food Sci. Nutr. 32: 818–824 (2003)

    Article  CAS  Google Scholar 

  15. Lee YL, Kim SH, Choung NH, Yim MH. A study on the production of viscous substance during the cheonggukjang fermentation. J. Korean Agric. Chem. Soc. 35: 202–209 (1992)

    Google Scholar 

  16. Choi SH, Park JS, Whang KS, Yoon MS, Choi WY. Production of microbial biopolymer, poly(γ-glutamic acid) by and Bacillus subtilis BS62. Agric. Chem. Biotechnol. 47: 60–64 (2004)

    CAS  Google Scholar 

  17. Meerak J, Yukphan P, Lida, Miyashita M, Sato H, Nakagawa Y, Tahara Y. Phylogeny of γ-polyglutamic acid-producing Bacillus strains isolated from a fermented locust bean product manufactured in west Africa. J. Gen. Appl. Microbiol. 54: 159–166 (2008)

    Article  CAS  Google Scholar 

  18. Lee MY, No HK, Kim SD, Prinyawiwatkul W. Quality of cheonggukjang prepared with various Bacillus strains. Int. J. Food Sci. Tech. 42: 587–592 (2007)

    Article  CAS  Google Scholar 

  19. Priest FG. Extracellular enzyme synthesis in the genus Bacillus. Bacteriol. Rev. 41: 711–753 (1977)

    CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Food Science and Technology, Kongju National University, Yesan, Chungnam, 340-802, Korea

    Joo-Hee Yang, In-Su Kim, Chul-Hwan Oh & Nam-Soon Oh

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  1. Joo-Hee Yang
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  2. In-Su Kim
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  3. Chul-Hwan Oh
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  4. Nam-Soon Oh
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Correspondence to Nam-Soon Oh.

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Yang, JH., Kim, IS., Oh, CH. et al. Protease and γ-glutamyltranspeptidase activities of bacillus spp. isolated from rice straw. Food Sci Biotechnol 21, 291–294 (2012). https://doi.org/10.1007/s10068-012-0039-8

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  • DOI: https://doi.org/10.1007/s10068-012-0039-8

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